Then, the suspension was centrifuged (1200 rpm, 7 min) and the supernate
The activities of ATPases and GSH content in supernate
were detected according to the instructions of kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
DNA ploidy of bladder cancer using bladder biopsy supernate
Total cholesterol was evaluated by an enzymatic method (Abbott Diagnostics) and high density lipoprotein cholesterol was determined in the supernate
after precipitating the other lipoproteins with phosphotungstic acid (Sigma Diagnostics).
Briefly, about 4 ml of of urine was centrifuged 2000xg for 15 min and pellet was treated with universal solution as per the package insert supplied in the kit and after boiling step the supernate
was used for doing ELISA.
After spinning at x 14,000g, the supernate
was transferred to new tubes and extracted twice with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) using the Eppendorpf Phase-Lok system (Westbury, NY).
Second (Jun 2005) Supernate
cocktail from first infectivity trial (P1), strains 149 and flag2 tested alone (P0 stool samples).
The acellular supernate
from the first lavage was saved separately from subsequent lavages for analysis of lactate dehydrogenase (LDH) activity and protein content.
After tissue removal by filtration through a double cheesecloth layer, the egg suspension passing through was allowed to settle ([approximately equal to]20-30 min), the supernate
was removed, and the eggs were washed twice more by resuspension and settling.
When Cooper next decanted the digesters, about a week into the test, he was able to decant more and the supernate
was clearer than before.
For virus isolation, frozen tissues were homogenized, the supernate
filtered through a 0.
Though a harsh extraction procedure, previous bioassays indicated (i) that the biologically active compounds remained in tissue residual when extracted with cold methanol, (ii) that some of the biologically active compounds remained in the tissue residual and some were captured in the supernate
when extracted with room temperature methanol for 24 h, and (iii) that the biologically active compounds were pulled from the tissue residual and captured in the supernate
when exposed to boiling methanol by the modified Coseteng and Lee method presented above (Warnock, 1998).
The bursa homogenate was centrifuged at 1,500 xg for 20 minutes, and the supernate
was collected and stored frozen at -70[grados]c until it was used as inoculum.
010 Osmolarity Fixed near 287 mOsm Ammonium sulfate precipitate test Supernate
will be red-brown; reagent test strip will be positive for blood.
75-77] These unbound remnants remain in the supernate
and have been identified as remnant-like particles (RLP).