The activities of ATPases and GSH content in supernate
were detected according to the instructions of kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
AM-conditioned media were collected and centrifuged, and the supernates
were saved in aliquots at -80[degrees]C for further analysis of cytokines.
Manufacturers have retained Lp(a) in LDL supernates
to mimic the beta quantification LDL.
The remaining polymers were cleaned by dissolution of the polymer in THF, centrifugation of the solutions at 4000 rpm ([similar to] 2000 g force), and precipitation of the supernate
solution into methanol, a non-solvent.
The fermentation broth was suspended and the supernate
was absorbed with the rice chaff.
The acellular supernate
from the first lavage (6 mL) was saved separately from the subsequent lavages for further analysis of lactate dehydrogenase (LDH) activity and protein content.
prec] was calculated as the difference between the TC in serum and the supernate
was decanted, and pelleted specimens were stored at 4 [degrees] C until subjected to five freeze-thaw cycles, followed by phenol-chloroform extraction to extract DNA.
coli MPN (most probable number) in 100 mL of the supernate
was determined using the Colilert-18 system (IDEXX Laboratories, Westbrook, Maine); incubation temperature was 35[degrees]C.
R&D Systems has developed a multiplexed assay that simultaneously measures multiple cytokines in a single cell culture supernate
Although the separation step was performed manually, the required equipment was readily available and cholesterol in the supernate
could be easily measured by various automated analyzers.
We believe that controlling the entire process, from clone supernate
as a source of raw material to finished reagent, will allow us to maintain the quality product and service for which Gamma is known.
The cholesterol concentration was measured in duplicate in the bottom fraction and in the HDL supernate
prepared from the bottom fraction (to meet the requirements of the CDC comparison design) by the dextran sulfate [MT 50 000 method of Warnick et al.
After 10 min at room temperature, the mixture was centrifuged for 10 min at 15008; 50 [micro]L of the supernate
was pipetted into a test tube, and 0.
The mixture was centrifuged, and an aliquot of the supernate
(50 [micro]L) was applied to a disk of cotton fiber placed in a well of a second microtiter plate as described.