coli O157:H7 from the supernatant
, prepared as described above, was diluted in HEPES-buffered saline and then passed over anti-O157 antibody-coated sensors for 10 min at a flow rate of 20 [micro]L/min.
were removed and brought up to 500 [micro]L with the tissue lysis buffer and incubated rocking overnight at 4[degrees]C with 5 [micro]g rabbit anti-mouse SF-1 (Upstate Biotech, Lake Placid, NY) per 0.
Cell culture supernatants
were stored in plastic containers at -80[degrees]C until analysis.
was harvested 48 h and 72 h posttransfection, filtered through 0.
For these samples, we used boiled blood supernatant
as the DNA source (9).
Four milliliters of the supernatant
were collected as the throat wash supernatant
was collected and evaporated under nitrogen gas.
Currently, hybridoma selection from a mixed pool of cells involves limiting dilution cloning and assay of culture supernatants
for antigen specificity of secreted antibodies.
Here we describe a procedure for the determination of Kyn and Trp concentrations in human plasma or in supernatants
of human cell cultures.
This indicated that the 20-kDa band was a specific byproduct of supernatant
protease activity, present in all supernatants
(except for strains M61 and 313).
were saved and stored at -70 [degrees] C for at least 30 min before analysis.
RBM's MAPs are used today for retrospective studies of banked samples, prospective studies on ongoing clinical trials, post-drug approval surveillance monitoring, timed studies of a single mouse, correlation of a subject's genotype to phenotype, basic research on tissue culture supernatants
, studies comparing gene expression to MAPs, animal model phenotyping and qualifying clinical trial participants.
The amounts of fetal DNA in the supernatants
cleared by ultracentrifugation were approximately fourfold higher than those in the matching STBM preparations.
Viral RNA was extracted from 200/[micro]L of cell culture supernatants
or NPA specimens by using the QIAamp viral RNA Mini Kit (QIAGEN, Inc.
The mixture was then centrifuged twice at 4 [degrees] C and supernatants