and deposits from the deep sea area and the fronts of the e2, E6 and e7 excavators of the heart field mine.
The obtained suspension was centrifuged at 10000 rpm for 10 min and the supernatant
was filtered through Whitman paper number 1 (Akbar and Javaid 2012).
Further the cultures were divided into three parts, the first part was centrifuged at 10,000 rpm at 4 degC for 10 min and its supernatant
and the supernatant
of each flask was separated, and then sterilized with 0.
Algicidal activity effect at different volume of filtrated culture supernatant
of Gb03 on C.
2) They reported fluid accumulation in the suckling mouse assay, vacuolating activity on cell culture assays, induced by thermolabile culture supernatant
, since it lost activity after being heated at 60[degrees]C for 15 min.
Then the supernatant
was directly used for the determination of chymosin activity.
After 60 minutes contact time the supernatant
liquid from each conical flask was collected through syringes in the time interval of 10 minutes.
Effects of Malvidin and Its Glycosides on SOD Production in Supernatant
After centrifuging the culture at 10,000 g for 10 min, supernatant
was collected and filtered through a 0.
Therefore, this research aimed to analyze whether the culture supernatant
derived from T.
Following centrifugation, the supernatant
was transferred to a tube and 900 [micro]L ice-cold 95% ethanol was added.
was collected, and the solid substance was removed.
Such preparations consisted of the supernatant
and the fungal extract obtained by sonication.
Two variants of reaction mixture were prepared for MSNS production, one with culture supernatant
and another with the harvested cells.