subtilis produced more than 50 percent inhibition of radial growth of pathogens where in this inhibition was experimentally attributed to the release of several antifungal metabolites such as
subtilin, bacitracin, bacillin and bacillomycin (Moubarak and AbdelMonaim 2011).
Subtilin is a small peptide antibiotic of 32 amino acids produced by B.
Muaz and Shahida, [5] in a research discovered the production of bacitracin and
subtilin by Bacillus sp.
They found secretion of antimicrobials; gramicidin, gatavalin, polymxin,
subtilin, and tyrocidine by biofilms in continuous reactors.
But notable exceptions to this rule are nisin and
subtilin which exhibit bacterial activity against gram-positive bacteria.