The difference between BsaJI and BseDI restriction enzyme
is the incubation time for the digestion.
The restriction endonucleases AseI, MaeI, MboII, TaqI, and BsmAI were selected for the first PCR product (for the 709-bp amplified product using primers LCO1490 and HC02198), and the restriction enzymes
BsaJI, NlaIV, MaeII, TfiI, and BfaI were selected for the second product (for the 597-bp amplified product using primers rntd-10 and C/N-2769) based on the best discrimination and species-specific amplicon patterns.
and expected fragment sizes Restriction Expected DNA SNP Enzyme fragments M680I Hinf I Wild; 126 bp+234 bp Mutant; 360 bp M694V Hph I Wild; 195 bp+23 bp Mutant; 218 bp M694I Pag I Wild; 195 bp Mutant; 182 bp+13 bp V726A Alu I Wild; 360 bp Mutant; 320 bp+40 bp SNP: single-nucleotide polymorphism; bp: base pair Table 2.
dagger]) Defined by pulsed-field gel electrophoresis XbaI restriction enzyme
2], [epsilon]) which pops the symbol from the stack acts by using also the restriction enzyme
Bg/I (Appendix 3).
This methodology can also be adopted for identification of mutations, if point mutations cause any creation or loss of restriction enzyme
Although linker-mediated amplification has been in use for many years (5) and its successful use in conjunction with a methylation-sensitive restriction enzyme
digest has been described as well (6), we found that a simple restriction enzyme
digest followed by linker ligation and PCR yielded insufficient product for downstream microarray analysis when starting with small samples.
In the same way, a restriction enzyme
scans a large DNA molecule (analogous to the textbook) to find its particular recognition site (analogous to the word "house").
Conformation of transformation by restriction enzyme
multiple OTUs possible for a single fragment size) and variation in restriction enzyme
efficacy may make the TRFLP technique less effective for use with gross measures of community structure such as diversity and richness.
All isolates were digested with the restriction enzyme
XbaI and subjected to pulsed-field gel electrophoresis (PFGE).
Since these abnormal hemoglobins reside on the same codon, they are also mixed with procedures like the Eco RI restriction enzyme
protocols at the gene level.
25) Meselson, M and R Yuan, 'DNA Restriction Enzyme
The method utilizes an oligonucleotide primer that contains a recognition site for a restriction enzyme
such that digestion with the restriction enzyme
generates a 5' overhang containing the locus of interest.
The restriction enzyme
Dde I can delineate the two species; however, when we discovered the ambiguity, no samples remained for analysis.