We found that gDNA within TDCPP-treated embryos--but not vehicle-treated embryos--at the end of cleavage was completely digested by methylation-sensitive restriction endonucleases
, suggesting that normal gDNA methylation was absent in TDCPP-treated embryos at 2 hpf.
For over ten years, the vast majority of molecular methods that analysed variation in DNA sequence relied on the special properties of the second major discovery, restriction endonucleases
Five (Cfo I, Sau 96 I, Hpa II, Hin f I and Alu I) of the 12 restriction endonucleases
cleaved the amplicons with more than one haplotype.
Digestion of amplified allele products with a restriction endonuclease
can also provide very discrete typing results.
DNA polymorphism detectable by restriction endonucleases
2008) greatly facilitated optimization of the key by allowing us to perform virtual restriction endonuclease
digestion (VRED) analyses, which saved time by eliminating much of the laboratory-intense trial-and-error usually associated with identifying informative restriction endonucleases
For example, Methanococcus jannaschii produces a specific restriction endonuclease
found to be useful in cleavage of small single-stranded DNA "flaps.
Also molecular property screening methods, including a preferred method, termed end selection, comprised of using an enzyme, such as a topoisomerase, a restriction endonuclease
, &/or a nicking enzyme (such as N.
Although numerous methods can be used for genotyping HCV, only the line-probe assay, nucleic acid sequence analysis, restriction endonuclease
cleavage, and cleavase fragment length polymorphism (CFLP) will be discussed here.
These data were obtained from restriction endonuclease
digestions of PCR products that included over 97% of the mtDNA genome and resolved 16 different haplotypes in 258 fish from 13 locations.
jeikeium genomic DNA which was treated with a restriction endonuclease
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All 3 isolates were identical when tested by random amplification of polymorphic DNA (6) and by pulsed-field gel electrophoresis after restriction endonuclease
digestion of chromosomal DNA with SpeI.
The preferred approach is genotyping (1), which is commonly performed by macrorestriction digestion of genomic DNA with a rare-cutting restriction endonuclease
analysis (REA): 10 [micro]g of genomic DNA was digested with 100 units each of Hind III, Hae III and EcoRI restriction enzymes (Boehringer Mannheim, Germany) according to the manufacturer's instruction.