restriction endonuclease


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Synonyms for restriction endonuclease

any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments

References in periodicals archive ?
We found that gDNA within TDCPP-treated embryos--but not vehicle-treated embryos--at the end of cleavage was completely digested by methylation-sensitive restriction endonucleases, suggesting that normal gDNA methylation was absent in TDCPP-treated embryos at 2 hpf.
For over ten years, the vast majority of molecular methods that analysed variation in DNA sequence relied on the special properties of the second major discovery, restriction endonucleases.
Five (Cfo I, Sau 96 I, Hpa II, Hin f I and Alu I) of the 12 restriction endonucleases cleaved the amplicons with more than one haplotype.
Digestion of amplified allele products with a restriction endonuclease can also provide very discrete typing results.
2008) greatly facilitated optimization of the key by allowing us to perform virtual restriction endonuclease digestion (VRED) analyses, which saved time by eliminating much of the laboratory-intense trial-and-error usually associated with identifying informative restriction endonucleases.
For example, Methanococcus jannaschii produces a specific restriction endonuclease found to be useful in cleavage of small single-stranded DNA "flaps.
Also molecular property screening methods, including a preferred method, termed end selection, comprised of using an enzyme, such as a topoisomerase, a restriction endonuclease, &/or a nicking enzyme (such as N.
Although numerous methods can be used for genotyping HCV, only the line-probe assay, nucleic acid sequence analysis, restriction endonuclease cleavage, and cleavase fragment length polymorphism (CFLP) will be discussed here.
These data were obtained from restriction endonuclease digestions of PCR products that included over 97% of the mtDNA genome and resolved 16 different haplotypes in 258 fish from 13 locations.
jeikeium genomic DNA which was treated with a restriction endonuclease.
com and titled Reagents Market by Application (Cloning, Epigenetics, PCR, Restriction Digestion, Sequencing), Product (Ligase, Phosphatase, Polymerase, Protease, Restriction Endonuclease, Reverse Transcriptase) - Global Forecast to 2018 says key drivers for this market are the rising R&D expenditure by the pharmaceutical and biotech companies, and increasing public funding for life science research.
All 3 isolates were identical when tested by random amplification of polymorphic DNA (6) and by pulsed-field gel electrophoresis after restriction endonuclease digestion of chromosomal DNA with SpeI.
The preferred approach is genotyping (1), which is commonly performed by macrorestriction digestion of genomic DNA with a rare-cutting restriction endonuclease (2,3).
Restriction endonuclease analysis (REA): 10 [micro]g of genomic DNA was digested with 100 units each of Hind III, Hae III and EcoRI restriction enzymes (Boehringer Mannheim, Germany) according to the manufacturer's instruction.
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