reaction, a neglected microbiological technique.
1933, Kristal-lstruktur und Quellung
pneumoniae is based on the hemolysis pattern when cultured on blood agar plates and by additional biochemical tests on the cultured organisms: optochin sensitivity, the Quellung
reaction, and bile solubility .
Typing antisera factors 29b and 35c are the CDC Quellung
reagents definitive for serotype 35B.
The pleural fluid culture and hemoculture of the patient yielded penicillin-susceptible Streptococcus pneumoniae and the isolate was identified as serotype 9V by the Quellung
reaction using serotype-specific antisera (Statens Seruminstitut; Copenhagen, Denmark).
15,16) The isolates with negative or equivocal results in multiplex-PCR were sent to Adolfo Lutz Institute (National Reference Laboratory, Ministry of Health) and subjected to Quellung
reaction for definition of capsular serotype.
The nine study participants provided the results of the isolate serotyping, which was done using the quellung
reaction with antisera produced by the Statens Seruminstitut of Copenhagen, Denmark (12), as well as the data on antimicrobial susceptibility to penicillin, ceftriaxone, vancomycin, erythromycin, tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole (TMP-SMX), which were determined by both the disk diffusion method (Kirby-Bauer) and broth microdilution, according to National Committee for Clinical Laboratory Standards (NCCLS) (13) (now Clinical and Laboratory Standards Institute, CLSI).
Serotyping was performed at Boston University Medical Center, using the Quellung
reaction with pneumococcal antisera.
1942, Kristalstruktur und innerkristalline Quellung
der Montmorillonits, Z.
Pneumococci were serotyped by the Quellung
method (Statens Serum Institut, Copenhagen, Denmark).
Las tecnicas para determinar el serotipo capsular se basaron en la reaccion de Quellung
, con el uso de antisueros procedentes de Statens Serum Institut, de Dinamarca (25, 26).
Pneumococcal isolates were sent to reference laboratories for serotyping by the quellung
reaction and were categorized as vaccine-type (VT) (serotypes included in PCV7) or nonvaccine-type (NT) (all other serotypes).
Serotypes were determined by the capsular quellung
reaction by using antiserum purchased from the Statens Serum Institute (Copenhagen, Denmark).