112] isolated and cultured protoplasts
of wild and cultivated sunflower and obtained roots and meristematic regions from protoplast
In vitro regeneration and protoplast
Generation of superoxide anion by potato tuber protoplast
during hypersensitive response to hyphal wall components of Phytophtora infestans and specific inhibition of the reaction with suppressors of hypersensitivity.
2] concentration may affect the regeneration potential of plant protoplast
In secretory tapeta the cells remain in situ until their degeneration, while in amoeboid tapeta the tapetal protoplasts
move into the loculus, forming a periplasmodium that is in direct contact with the developing microspores.
Contact with adjoining strips seals the endodermis as a whole, and thus water and dissolved substances must enter the protoplast
, which to some extent controls what passes in and out of the stele.
To broaden the genetic base of the common potato gene pool and to combine different resistance genes introgressed from wild potato species, various methods have been used, including ploidy manipulations and bridge crosses, embryo rescue, hormone treatments, reciprocal crosses and protoplast
fusion (Jansky, 2006).
It blew my mind when I combined Bacillus subtilis and Bacillus thermophilus via protoplast
fusion and made a new living species for my thesis.
Isolation of resistant mutants for a green alga Haematococcus pluvialis, and their hybrid formation by protoplast
fusion for breeding of higher astaxanthin producers.
On the basis of experiment with single, non-statolith containing cells, we developed a new model for plant gravity sensing in which we suggest that the entire protoplast
acts as the gravity sensor.
production and isolation from Etlingera elatior.
Among specific topics are the protoplast
transformation of filamentous fungi, developing Impala-based transposon systems for tagging genes in filamentous fungi, identifying differently expressed fungal genes in planta by suppression subtraction hybridization, applying laser micro-dissection to study plant-fungal pathogen interactions, measuring protein kinase and sugar kinase activity in plant pathogenic Fusarium species, and evaluating anti-fungal susceptibility using flow cytometry.
The two methods used in the lab are protoplast
formation and spore germination.
Japan Science and Technology Corporation (Saitama, Japan), National Institute of Agrobiological Sciences (Ibaraki, Japan), and Forestry and Forest Products Research Institute (Ibaraki, Japan) have patented a method for producing non-cellulosic callose fiber by using plant protoplast
, which imposes less burden to the environment with reduced energy consumption compared to conventional natural fibers is provided; it comprises the addition of an inorganic ion to a plant protoplast
cultivation system, which leads the plant protoplast
to produce non-cellulosic callose fiber.
preparation and regeneration from spores of the biocontrol fungus Pseudozyma flocculosa.