By the simultaneous application of two SgRNA, there was a large and significant deletion made of up to 245 kb in the chromosome in rice protoplasts
High efficiency colony formation and whole plant regeneration from mesophyll protoplasts
of Pelargonium X hortorum 'panache sud'.
Transformation of sugarcane protoplasts
by direct uptake of a selectable chimeric gene.
Cell suspensions are also suitable for protoplast
isolation in studies of transitory genetic expression and transformation by Agrobacterium (Torabi et al.
harzianum derived by protoplast
fusion (THVxTh291) is also able to inhibit in vitro colony growth of P.
Influence of donor material and genotype on protoplast
regeneration in banana and plantain cultivars (Musa spp.
have used EMS mutagenesis and interspecific protoplast
fusion to isolate improved probiotic yeasts.
Establishment of embryogenic callus and high protoplast
yielding suspension cultures of sugarcane (Saccharum spp.
cultivar "Bluggoe", ABB group) protoplast
isolated from regenerable embryogenic cell suspension.
Among specific topics are the protoplast
transformation of filamentous fungi, developing Impala-based transposon systems for tagging genes in filamentous fungi, identifying differently expressed fungal genes in planta by suppression subtraction hybridization, applying laser micro-dissection to study plant-fungal pathogen interactions, measuring protein kinase and sugar kinase activity in plant pathogenic Fusarium species, and evaluating anti-fungal susceptibility using flow cytometry.
Light and electron microscopic studies of protoplast
development during Entomophaga grylli pathotype 2 infections in Melanoplus differentialis.
The use of cellulases in animal feed, fruit processing, textile wet processing, preparation of dehydrated vegetables, food products, essential oils, flavours, starch processing, botanical extract, pulp and paper production, jams, juices, production of plant protoplast
for genetic manipulation, wine production and pharmaceutical and biomass conversion have greatly increased the prospects of enzymatic hydrolysis over chemical processes (Mach, and Zeilinger, 2003, Mekala et al.
The two methods used in the lab are protoplast
formation and spore germination.
Somatic hybrids obtained by protoplast
fusion between Solanum tuberosum L.
Japan Science and Technology Corporation (Saitama, Japan), National Institute of Agrobiological Sciences (Ibaraki, Japan), and Forestry and Forest Products Research Institute (Ibaraki, Japan) have patented a method for producing non-cellulosic callose fiber by using plant protoplast
, which imposes less burden to the environment with reduced energy consumption compared to conventional natural fibers is provided; it comprises the addition of an inorganic ion to a plant protoplast
cultivation system, which leads the plant protoplast
to produce non-cellulosic callose fiber.