One of the receptor molecules transporting lysosome components is the mannose-6-phosphate receptor (M6P receptor) (see figure 2), which recognizes and binds proteinase
precursors during their transit to the lysosome.
To determine whether the presence of a protein-digesting enzyme, proteinase
K, and a DNA coprecipitant significantly increased DNA yield, a pooled mouthwash sample was prepared.
6]-dependent enzymes, proteinases
in all four subclasses, phospholipases, kinases and transglutaminases, were among the drug targets encompassing numerous therapeutic areas, i.
The molecules turn on genes that instruct the plant to begin making proteinase
Extracellular BGPT1-10P and BGPT1-78 proteinases
have a molecular mass of about 29 kDa.
1996) and activation of intramuscular proteinase
, cathepsins (Ohmori et al.
Indeed, P1G10 displays 3-5-fold higher proteinase
activity than commercial papain, depending on the supplier.
Elevated aspartic proteinase
secretion and experimental pathogenicity of Candida albicans isolates from oral cavities of subjects infected with human immunodeficiency virus.
In this study we demonstrate that the addition of proteinase
K to the extraction procedure improves the yield of RNA from primary breast tumors, making the majority of samples eligible for array analysis by Qiagen RNeasy Minikit.
This inactivation was nonreversible and was similar to a pretreatment with a broad-spectrum protease (100 [micro]g/mL proteinase
K) for [is less than] 2 hr at 37 [degrees] C.
He covers blood, plasma, cells, and other biological fluids; plasma fractionation and plasma protein products; methods for analyzing plasma and plasma protein fractions; albumin; plasma immunoglobulins; factor VIII and von Willebrand factor; plasma proteinase
inhibitors; proteins dependent on vitamin K; and miscellaneous plasma proteins.
32) is a major cysteine proteinase
, isolated from pineapple (Ananas comosus) stem.
Through the development of our protocol, we show that proteinase
K was an ineffective cell lysis method, whereas sarcosyl efficiently lysed cells and could be used when its final assay concentration was diluted to avoid interference with dye fluorescence.
3]) were lysed in 50 [micro]L 1x PCR buffer containing 1% polyoxyethylene 10 lauryl ether and 200 [micro]g/mL proteinase
K at 56[degrees]C overnight.
The abnormal, or rogue, prion protein detected by these tests is considered to be the best indicator of TSE infection (2), but the anti-prion protein antibodies used in these assays cannot distinguish the normal, globular, proteinase
K-sensitive ([alpha]-helix-rich) form of the prion protein ([PrP.