These slides were observed under microscope (Olympus BX 41) (oil immersion) for the presence of micronuclei in 2000 polychromatic erythrocytes (Fig.
In in vivo micronucleus assay, the per cent micronucleated polychromatic erythrocytes (% MnPCE) per 2000 PCE was calculated in all the drug treated groups.
Percentage of micronucleated polychromatic erythrocytes (MnPCE in 2000PCE) and PCE (in 200 NE) in acute and subacute studies Treatment groups Acute study n=5 % MnPCE %PCE Normal saline 0.
The following parameters were analyzed in each specimen: a) the frequency of PCE among the first 100 NCE observed in each slide (PCE/NCE ratio); b) the frequency of micronucleated polychromatic erythrocytes
(MNPCE) within 2,000 polychromatic erythrocytes
counted; c) the frequency of micronucleated normochromatic erythrocytes (MNNCE) within 2,000 normochromatic erythrocytes counted; d) the frequency of dead cells (DC; erythrocytes with [is greater than] 3 micronuclei in the bone marrow) within 2,000 cells; e) the frequency of micronucleated peripheral blood erythrocytes (MNPBE) within 4,000 erythrocytes; f) the frequency of metaphases among 2,000 cells (MI); and g) the frequencies of cells with chromosome aberrations among 100 metaphases.