The current study was planned to correlate the results of frozen section and the final diagnosis on paraffin sections
in gynaecological neoplasms in order to document the efficacy of intra-operative frozen section.
An immunohistochemical evaluation of progesterone receptor in frozen sections, paraffin sections
, and cytologic imprints of breast carcinomas.
Nevertheless, paraffin section
IHC stains for [kappa], [lambda],and sometimes the immunoglobulin heavy chains are useful in evaluating potential lymphoid or plasmacytic neoplasms, especially if there is plasmacytoid differentiation or overt plasma cells.
The interpretation of staining or its absence was confirmed by subsequent IHC on paraffin sections
together with positive controls.
In paraffin sections
, myeloperoxidase and lysozyme antibodies have been used as sensitive and specific markers for cells of myeloid lineage?
Immunohistochemical testing was performed on paraffin sections
using rabbit antibody to CD103 (clone EPR4166, Abcam, Cambridge, Massachusetts) and mouse monoclonal antibody to Pax5 (clone 1EW, Leica Microsystems, Buffalo Grove, Illinois).
of the skin, oral mucosa, duodenum, pancreas and mesenteric lymph nodes were stained with Grocott's methenamine silver stain (GMS) for the identification of Pythium insidiosum.
The paraffin sections
were stained with hematoxylin and eosin.
Two weeks later, the mice were sacrificed; part of the aortic tissue was used to prepare paraffin sections
and the other part was analyzed as a homogenized supernatant.
In few paraffin sections
, very weak staining was observed that could be due to prolong fixation in formalin (Faizee et al.
Moreover, these particular kits (not originally developed for FFPE tissues) have also been frequently used for nucleic acid extraction from FFPE tissue specimens, some employing innovative modifications of the original extraction procedure, such as pretreatment of paraffin sections
with elevated temperatures (9), melting of paraffin directly in tissue lysis buffers (10), and/or addition of a reverse formalin cross-linking step (10).
The aim of our study was to assess the usefulness and accuracy of cytologic smears by making squash preparation as a means of intraoperative diagnostic method in the neurosurgical lesions and its correlation with histopathological diagnosis on paraffin sections
The mucosae were put in 10% neutral formalin solution for 1 day and prepared into 5-7 m thick paraffin sections
which were subjected to hematoxylin- eosin staining.
The unmasking of antigen in paraffin sections
of tissue by trypsin.
DNA recovered from tissue in paraffin sections
was successfully amplified and detected with this assay (data not shown), confirming the presence of C.