Parts of a gill of this bivalve were removed, washed with seawater, postfixed in 2% osmic
acid in seawater, dehydrated in ethanol, embedded in Spurr resin, sectioned and examined as for the Middle Valley specimens.
The system is composed of 3rd generation Osmic
VariMax optics, the Saturn 944 CCD detector, and the AFC-Kappa goniometer.
1 M cacodilate solution with 1% osmic
tetroxide for 1 to 2 h.
OSMIUM tetroxide also known as osmic
acid is a catalyst used in labs around the world.
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For better identification of nervous tissue, we used an osmic
acid fixation and examined whole preparations and serial semithin sections.
Beijing, China); (3) fixing in osmic
acid (Alfa Aesar, Massachusetts, USA) for 1.