It was ligated
and cloned as pTZ-HBVc, restricted with EcoRI and NdeI and ligated
in to pET22b (+) plasmid restricted with the same enzymes.
In a surgeon's knot, configuration, the cystic duct is ligated
intracorporeally as described by Nathanson and Colleagues.
Bleeding branches of main vessels, and vessels supplying the areas having good collateral circulations were ligated
We could suppose that the peripheral vascular branches might be ligated
during the repeated surgical exploration, then new blood supply might grow either from the external or ICA.
Results: Extracellular domain of ictla-4 gene was ligated
to igg1 gene and then ictla4-ig fragment was cloned into pBudCE4.
The 5,402 nt SINV-3 fragment and the linearized pFastBac1 vector were gel purified and ligated
for 18 h at 16[degrees]C.
2,5) A transected bile duct draining a segment or a smaller part of the liver can be safely ligated
before it becomes colonized with bacteria and fungi.
The vessels around the area were ligated
with 2/ 0 chromic catgut and subsequently the mucocele was removed.
The authors themselves indicate this problem "it is first important to know which side is ligated
first, since there is degeneracy in the stack side .
Ehasani et al  in their recent study compared the amount of expressed frictional resistance between orthodontic self-ligating brackets and conventionally ligated
The blood supply to the uterus is then ligated
, and the organs are taken from the animal's abdomen.
In addition, Student's-t test was used for intragroup comparisons between ligated
and unligated teeth.
COMPARISON OF RESISTANCE TO SLIDING PRODUCED BY SELF-ILIGATING BRACKETS AND CONVENTIONAL BRACKETS LIGATED
WITH CONVENTIONAL ELASTOMERIC LIGARATURE AND LOW-FRICTION LIGARATURES
The fistula at the base of right coronary artery which was extending to the right ventricle was ligated
with pledgeted suture of 5/0 prolene.
Then we ligated
our gene into a pET plasmid that also contained a gene for resistance to ampicillin.