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protein separation by isoelectric point followed by separation by molecular weight followed by mass spectrometry.
after the maximum) can be due to the decreased electrostatic repulsion and increased casein-casein interactions as caseins approach their isoelectric point (Lucey, 2004).
Escudey M, Galindo G, Ervin J (1986) Effect of iron oxide dissolution treatment on the isoelectric point of allophanic soils.
The labeled protein spots covered a wide range of relative molecular masses and isoelectric points.
Sequence analysis by the top-down approaches tends to involve more laborious analysis of one protein at a time, although characteristics of the protein such as molecular size and isoelectric point may be known.
Immunologic methods are convenient and timeefficient for routine use in central laboratories with high specimen throughput, but because these tests separate CDT from non-CDT moieties on the basis of differences in isoelectric point (pl), they will be disturbed by genetic transferrin polymorphisms (12) and by congenital disorders of glycosylation (13), which can cause falsely high or low results that may lead to false-positive or -negative identification of patients for alcohol misuse.
These strains all produced substantial amounts of AmpC [beta]-lactamase with the isoelectric point characteristic of the E.
coli isolates phenotypically suspected of ESBL production, a [beta]-lactamase with an isoelectric point of 8.
For the lanes representing samples whose rHuEPO content was unknown, band zero (0) is identified as the band with the same isoelectric point (pI) as the most acidic band in the rHuEPO marker.
8 (n=3) [greater than or equal to 32] (n=38) 18 (e) 2 16 (n=6) 5 (e) 0 8 (n=26) 12 (g) 0 4 (n=19) 6 (g) 1 (g) 2 (n=10) 2 (g) 0 [less than or equal to] 1 (n=11) 0 0 (a) pI, Isoelectric point.
Microbiologic and biochemical tests confirmed the presence of an extended spectrum beta-lactamase displaying an apparent isoelectric point value of 8.
The number of charged molecules, such as the sialic acid content of the carbohydrate, influences the isoelectric point (pI), which in turn determines the final position of the isoform on the gel.
Lysozyme is a small molecule (14-15 kDa) with a high isoelectric point (pI 10.
Transferrin isofocusing and other techniques that separate on the basis of isoelectric point are unable to discriminate between these two asialotransferrins, but the abnormal transferrin isofocusing pattern in S.