The dye adsorption was studied in a batch experiment with 50 mL aqueous solution of 5 mg/L basic fuchsin
and methyl violet dyes for 48 h at 25[degrees]C.
The fixed smears of (either wild caught or Wolbachia cured) were flooded with freshly filtered carbol fuchsin
solution prepared from 2 ml of stock solution of basic fuchsin
in 5 ml of phosphate buffer for 10 sec.
Histological pancreas islets were checked separately in hematoxylin and eosin with aldehyde fuchsin
5% basic fuchsin
for 24h at 37[degrees]C and then rinsed thoroughly in tap water.
The samples were then mixed with phenol ammonium sulphate basic fuchsin
solution and stored at ambient conditions.
5 mm-thick slices were cut with the diamond saw, polished, stained with toluidine blue and basic fuchsin
, and digitally imaged using a compound microscope with a combination of incident illumination and transillumination.
Samples were mounted in glycerin jelly containing phenol and basic fuchsin
, and examined with light microscopy.
Pollen was removed from the leg of eleven bees (family Apidae; ten bees from site 1, one bee from site 3) and placed, separately, into microcentrifuge tubes containing Calberla's stain (2 drops of saturated aqueous solution of basic fuchsin
, 5 ml glycerol, 10 ml 95% ethanol, and 15 ml distilled water).
Sections (2 [mu]m) for light microscopy were stained with methylene blue-azure II and basic fuchsin
(Humphrey et al.
Semithin (1 to 2 pm) sections were cut with glass knives on an ultramicrotome and stained with toluidine blue and basic fuchsin
for light microscopic examination with bright-field and differential interference contrast optics.
A few Gram negatives tend to stain faintly with safranin and are more effectively demonstrated using an alternate counter-stain, such as basic fuchsin
or carbol fuchsin
A) General Use -- Alcloxa, Alum (Potassium), Aluminum Sulfate, Amyltricresols (Secondary), Basic Fuchsin
, Benzethonium Chloride, Benzoic Acid, Benzoxiquine, Boric Acid, Camphor, Candicidin, Chlorothymol, Coal Tar, Dichlorophen, Menthol, Methylparaben, Oxyquinoline, Oxyquinoline Sulfate, Phenol, Phenolate Sodium, Phenyl Salicylate, Propionic Acid, Propylparaben, Resorcinol, Salicylic Acid, Sodium Borate, Sodium Caprylate, Sodium Propionate, Sulfur, Tannic Acid, Thymol, Tolindate, Triacetin, Zinc, Caprylate, Zinc Propionate.
This test is described in detail in the final regulation and basically involves pumping Syngyna fluid (a combination of sodium chloride, certified reagent acid fuchsin
and distilled water) into a tampon until the tampon is saturated.
The major components of the MMPS, as used in our laboratory, are Verhoeff elastic hematoxylin, sodium thiosulfate, acetic acid, and alcian blue, crocein scarlet-acid fuchsin
, and alcoholic saffron solutions.
Later, all sandflies were stained with 20% carbol fuchsin
solution and then identified to species by microscope-based on some typical morphologic characteristics: mainly internal structures (such as hair on abdominal tergites, buccal capsule, pigment patch, pharyngeal basket and spermatheca, ciborium, pharynx for females and terminal genitalia for males).