Comparison of Chemicon SimulFluor direct fluorescent antibody
staining with cell culture and shell vial direct immunoperoxidase staining for detection of herpes simplex virus and with cytospin direct immunofluorescence staining for detection of varicella-zoster virus.
The RIDT is a practical tool for field application to gather surveillance data of rabies-suspect animals, especially in resource poor regions where fluorescent antibody
testing is impractical.
Evaluation of the indirect fluorescent antibody
test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigsPesq.
Instead of labelling the protein of interest with a fluorescent antibody
, the team coated it onto smaller beads (two [micro]m in diameter) which are both magnetic and fluorescent.
2012) Evaluation of Indirect Fluorescent Antibody
Assays Compared to Rapid Influenza Diagnostic Tests for the Detection of Pandemic Influenza A (H1 N1) pdm09.
All the children had specimens tested via conventional methods (immunochromatography, direct fluorescent antibody
, shell vial, and tube culture) and then again by a more specific viral molecular assay test--nucleic acid tests (NAT).
Such differences in cellular attributes are most commonly determined by multicolor fluorescent antibody
detection of cell type specific surface marker(s) using flow cytometry.
Staining with a single fluorescent antibody
such as antiCD4 antibody tagged with Alexa Fluor 633 showed clear specific IF signals without AF, providing a simple and practical protocol for single color IF staining in renal tissues.
Older tests for Lyme disease include whole cell enzyme-linked immunoassay (ELISA), indirect fluorescent antibody
(IFA), Western blot, and C6 (SNAP* and ELISA).
The fluorescent antibody
technique (FAT) is simple, easy and quick and the results are more definite.
INDEX TERMS: Type A influenza, West Nile virus, rabies, pulsed field gel electrophoresis, real-time PCR, Mycobacteria growth indicator tube, Mycobacterium tuberculosis, multiplex PCR, direct fluorescent antibody
To find out whether Hv1 was the correct channel in sperm, the researchers added a green fluorescent antibody
stain to the channel and noted that Hv1 was abundant.
Use of a blue-excitable (Alexa 488) or a green-excitable (Alexa 555) fluorescent antibody
with a red-excitable one (Alexa 633) will provide complete spectral separation and accurate quantitation of both antibodies on one blot.
Direct fluorescent antibody
testing for respiratory viruses was positive in eight flocked swab samples and three nasopharyngeal aspirates.
techniques use the properties of dyes such as fluorescein and rhodamine to emit visible light in response to ultraviolet radiation (Figure 2-15).