Next, they added a solution of captopril with various concentrations to phosphate buffer of gold nanoparticles - fluorescein isothiocyanate
, and they studied the fluorescent spectrum of its emitted fluorescein isothiocyanate
5 for fluorescein isothiocyanate
, which covers most food products' pH.
Sites of probe hybridization were detected by applying 100 [micro]L of rabbit anti-biotin antibody (1:100 dilution with 2 mg/mL bovine serum albumin in 1x PBS) at 37 [degrees]C for 45 min, followed by using 100 [micro]L fluorescein isothiocyanate
(FITC) labeled goat anti-rabbit antibody (Enzo Diagnostics, New York; 1:100 dilution).
The D3 Duet(TM) DFA kits use brighter, easily distinguishable fluorescent stain colors to improve interpretation of virus-infected cell cultures by combining monoclonal antibodies labeled with either Fluorescein Isothiocyanate
(FITC) or R-Phycoerythrin (R-PE) in one reagent for simultaneous detection.
One set included GLP 13 (green) and GLP 21 (red), which were labeled by fluorescein isothiocyanate
and tetramethylrhodamine, respectively.
For single-color IF labeling, the sections were incubated with primary anti-CD4 antibodies (R&D Systems, Gymea, Australia) directly conjugated with Alexa Fluor 633 or anti-F4/80 conjugated with fluorescein isothiocyanate
(FITC; Invitrogen, Mulgrave, Victoria, Australia) in a humid environment at 4[degrees]C overnight.
2% normal sheep serum (Sigma) for 1 h followed by incubation with a diluted (1:100) solution of fluorescein isothiocyanate
(FITC)-conjugated anti-digoxienin anti body (Roche) for 2 h at 37[degrees]C.
After centrifugation at 400g for 5 min, we suspended the leukocytes in 490 [micro]L binding buffer (Annexin V-FITC Apoptosis Detection Kit, Biovision) and incubated the leukocyte suspension with 5 [micro]L each of reagent set-supplied, undiluted fluorescein isothiocyanate
-labeled annexin V (BioVision) and reagent set-supplied, undiluted propidium iodide (BioVision) at room temperature for 5 min in the dark.
After washing, these cells were first incubated with a monoclonal anti-insulin antibody (1:200 dilution; Sigma, Madrid, Spain) for 2 hr and then for 1 hr with a secondary anti-body, anti-mouse IgG-conjugated fluorescein isothiocyanate
(IgG-FITC; 1:200 dilution; Sigma), both at room temperature.
The fluorometer is used to measure the fluorescence signal of a suspension of microspheres with immobilized fluorescein isothiocyanate