fluorescein isothiocyanate

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Related to fluorescein isothiocyanate: FITC
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  • noun

Synonyms for fluorescein isothiocyanate

a fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence

References in periodicals archive ?
Next, they added a solution of captopril with various concentrations to phosphate buffer of gold nanoparticles - fluorescein isothiocyanate, and they studied the fluorescent spectrum of its emitted fluorescein isothiocyanate.
5 for fluorescein isothiocyanate, which covers most food products' pH.
5% Tween 20 in PBS) for 5 min each, and then exposed to goat anti-mouse IgG conjugated with fluorescein isothiocyanate (FITC) (1:100) (BOSTER, China).
After centrifugation at 400g for 5 min, we suspended the leukocytes in 490 [micro]L binding buffer (Annexin V-FITC Apoptosis Detection Kit, Biovision) and incubated the leukocyte suspension with 5 [micro]L each of reagent set-supplied, undiluted fluorescein isothiocyanate -labeled annexin V (BioVision) and reagent set-supplied, undiluted propidium iodide (BioVision) at room temperature for 5 min in the dark.
After washing, these cells were first incubated with a monoclonal anti-insulin antibody (1:200 dilution; Sigma, Madrid, Spain) for 2 hr and then for 1 hr with a secondary anti-body, anti-mouse IgG-conjugated fluorescein isothiocyanate (IgG-FITC; 1:200 dilution; Sigma), both at room temperature.
The fluorometer is used to measure the fluorescence signal of a suspension of microspheres with immobilized fluorescein isothiocyanate (FITC).
specific antibody CRY104 labeled with fluorescein isothiocyanate (Biotech Frontiers, North Ryde, Australia) for enumeration.
Secondary antibodies, all obtained from SigmaAldrich, were as follows: fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG ([gamma]-chain specific), peroxidase-conjugated anti-mouse IgG ([gamma]-chain specific), FITC-conjugated anti-rabbit IgG (whole molecule); and peroxidase-conjugated anti-rabbit IgG (whole molecule).