For immunoblot procedures, the particular recombinant antibodies were diluted using DMEM supplemented with 100 mL/L heat-inactivated fetal calf
serum and applied to the corresponding AlaBLOTs (F1, hen egg white; F2, bovine milk white; 11, whole bee venom; and F49, apple extract; DPC-Biermann) with native allergens, separated by SDS-PAGE, and immobilized onto nitrocellulose strips in the form of extracts or purified allergens.
For experiments, cells were subcultured into gelatin-coated 12-well plates in DMEM medium supplemented with glutamine (1 mM), penicillin (100 IU/ml), 10% fetal calf
serum and 10% human serum until subconfluency and confluency, in separated cultures, was reached.
In the past, the islets have all been isolated with fetal calf
Considering that the virus has been repeatedly detected in batches of fetal calf
serum (3,11), a possible introduction of the virus to the European continent through vaccines or other products prepared with contaminated bovine serum should be taken into account.
Aortic hVSMCs (Cambrex Bioscience Srl) cultured in minimum essential medium supplemented with 100 mL/L fetal calf
serum, 100 units/L penicillin, 100 [micro]g/L streptomycin, 10 mmol/L glutamine, and vitamins and buffered with 10 mmol/L TES and 10 mmol/L HEPES were incubated for 24 h with sodium azide at a final concentration of 76.
Briefly, we added the aliquots of the HPLC fractions (1-5 [micro]L) or HPLC-purified synthetic LTX-diol isomers in redistilled ethanol to cultured MCF-7 cells grown in phenol red-free Dulbecco's modified Eagle's medium (DMEM) containing 5% charcoal-stripped, sulfatase-treated fetal calf
5]) (Kiderlen and Kaye, 1990) in 200 [micro]l RPMI 1640 medium supplemented with 5% heat-inactivated fetal calf
serum (herein designated R5) were incubated for 48 h with test compounds (50 [micro]g/ml) or medium alone before further fetal calf
serum (10% final concentration) was added.
By comparison, genetically engineered products are extracted from the secretions from Chinese hamster ovary cells that are cultured in fetal calf
or other mammalian serum, and approximate human hormones.
Contract award: Delivery of laboratory glassware and fetal calf
After incubation for 24 h, monolayers of ECV304 were maintained in M199 medium containing 10% fetal calf
serum and observed daily with an inverted microscope.
Different amounts of testosterone and other androgenic compounds were added to charcoal-stripped fetal calf
serum, and the corresponding dose-response curves were obtained and compared with that of testosterone (Fig.
Cells were then centrifuged, resuspended in RPMI 1640 culture medium supplemented with 10% fetal calf
serum, 200 U/mL penicillin, 0.
Williams Medium E was obtained from Bio Whittaker (Verviers, Belgium), Dulbecco's MEM (1x) from Biochrom KG (Berlin, Germany) and fetal calf
serum from PAA Laboratories GmbH (Colbe, Germany).
CTFV can be stored at 4[degrees]C for 2-3 months in 50% fetal calf
Cells were grown in phenol red-free EMEM containing 2 mM glutamine, 1% nonessential amino acids, 100 U/mL antibiotics (mix of penicillin, streptomycin, and fungizone), 1 mM sodium pyruvate, and 10% fetal calf
serum (Biowhittaker, Gagny, France).