While many different cell lines have been published previously as feeder layer
(4), it has been reported that the murine fibroblast M210-B4 cell line can support the maintenance of LTC-IC from human bone marrow as effectively as standard human marrow adherent cells (5).
Thus human limbal epithelial cells isolated from human cadaveric donor eyes when co-cultured with 3T3 feeder layer
with denuded AM maintains the limbal epithelial cell properties.
The ES cells of mouse and human can be basically cultured on murine fibroblast feeder layers
with cytokine, leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF), respectively.
It was very difficult for pPB to attach to feeder layers
in Method A and only two of them attached.
Previous studies have shown that human limbal epithelial progenitor cells can be expanded and maintained on intact, but not denuded HAM or plastic in the absence of 3T3 murine fibroblast feeder layers
Briefly, ES cells were cultured in ES cell medium without the feeder layers
for 24-28 h, then the clumps were mechanically separated and cultured in suspension in LIF-free medium containing 15% FBS for 5-10 days until EBs formed.
Geron has also developed a culture system which allows the long term culture of hES cells in the absence of mouse feeder layers
(Xu et al.
Second, the Company is able to produce its cell lines without feeder layers
(layers of foreign cells used as an environment to grow the stem cells which can complicate the process and result in contamination of the stem cells).
The PGCs were cultured on feeder layers
and in media containing nutrients and specific growth factors.
In addition, PluriCells are cultured in a defined medium directly onto tissue culture plastic without the use of feeder layers
, making them ideally suited for commercial high-throughput research applications.
These PluriCell lines have been established directly onto tissue culture plastic without the use of feeder layers
or conditioned medium.
Those cells were cultured with the use of animal products and/or feeder layers
of non-human cells.
These PluriCell([TM]) lines have been established directly onto tissue culture plastic without the use of feeder layers
or conditioned medium.
These PluriCell lines can be efficiently cultured in tissue culture plates without the use of feeder layers
and can be efficiently differentiated into multiple cell-types, having the potential to aid in drug discovery and development.
The line of Lifeline products includes: standardized human "feeder" cells (called "fibroblasts") and specialized animal-free growth medium, a system that can provide feeder layers
to grow and maintain human embryonic stem cells without contamination from animal cells (sold under the name FibroLife[TM]); a kit containing human blood-vessel cells and growth media (sold under the name "VascuLife[TM]) for the study of cardiovascular diseases; and a unique line of adult nerve cells and nerve stem cells with the ability to survive in low-oxygen conditions, for research into stroke.