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Fecal DNA versus fecal occult blood for colorectal-cancer screening in an average-risk population.
Genomic DNA was isolated from influent and effluent aerobic digester samples using Promega's Wizard[R] Genomic DNA Purification kit, MO BIO Laboratories' UltraClean[TM] Microbial DNA Isolation kit, UltraClean[TM] Fecal DNA kit, and UltraClean[TM] Mega Soil DNA kit and detected by gel electrophoresis and ethidium bromide staining (Table 1).
Samples were refrigerated for 24-48 h before DNA extracts were prepared by using the revised protocol described in the ZR Fecal DNA Kit (Zymo Research, Irvine, CA, USA).
QIAamp Kit was used to purify fecal DNA (from enrichment and non-enrichment feces) and E.
Aberrantly methylated CDKN2A, MGMT, and MLH1 in colon polyps and in fecal DNA from patients with colorectal polyps.
Fecal DNA analysis represents a promising non-invasive approach to screen for mutations associated with colorectal cancer.
Fecal DNA testing detects advanced colorectal neoplasia in asymptomatic patients at average risk for colorectal cancer with a significantly greater sensitivity than fecal occult-blood testing, reported Thomas F.
This test analyzes fecal DNA and offers the ability to detect potential cancers at very early stages.
Stool DNA testing (also called fecal DNA testing ) is not currently recommended as a method to screen for colorectal cancer by the United States Preventive Services Task Force (USPSTF).
Covering the same potential mutations but using another assay, the largest study in the field, which was aimed at the evaluation of fecal DNA testing for the detection of colorectal neoplasms, reported a prevalence of only 1.
Fecal DNA testing as a screening method for colorectal cancer is less cost effective than other accepted screening methods in nearly all circumstances, but it could reduce the number of colonoscopies an average person would have to undergo, reported Dr.
1 mM concentrations each of 2'-deoxynucleoside 5'-triphosphate, 2 U of recombinant DNA polymerase (rTaq) (Perkin Elmer, Norwalk, CT), and 10 [micro]l purified fecal DNA.
Further work is needed to evaluate the specificity of the new marker panel and to validate the panel for screening fecal DNA.
The investigators analyzed the methylation status of 10 genes in the fecal DNA of 10 patients with colorectal cancer and 13 endoscopically diagnosed healthy control patients.