exonuclease


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  • noun

Words related to exonuclease

a nuclease that releases one nucleotide at a time (serially) beginning at one of a nucleic acid

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References in periodicals archive ?
In addition, chemically modified sgRNA may aid in editing challenging targets even in common cell lines, and can provide protection against exonucleases in prokaryotic cells.
Exonuclease treatment was performed manually the next day.
Clinicopathological analysis of endometrial carcinomas harboring somatic POLE exonuclease domain mutations.
The 3' phosphorylated primers were used for the digestion of dsDNA by lambda exonuclease in order to get ssDNA.
Avci-Adali M, Paul A, Wilhelm N, Ziemer G, Wendel HP (2010) Upgrading SELEX technology by using lambda exonuclease digestion for single-stranded DNA generation.
Lucigen's products include pSMART GC VectorspGC Blue VectorsBigEasy Long PCRCloneSmart, BigEasy Linear, DNATerminator End Repair Kit, CloneDirect Rapid Ligation Kit, UltraClone DNA Ligation and Transformation Kits, Gel-Ready DNA Ladders, NxGen T4 DNA Ligase, NxGen T4 RNA Ligase, NxGen Exonuclease I, NxGen Lambda Exonuclease, NxGen Exonuclease III, NxGen RNAse Inhibitor, RNase I, T4 Polynucleotide Kinase, and Pyrophosphatase, among others.
Nucleases catalyzing DNA and RNA cleavage are indispensable for life, nuclease activities are integral parts of DNA replication; the 5' to 3' exo-and endonucleases are needed to remove RNA primers, and the 3' to 5' exonuclease for proofreading (6).
Samples were digested for 45 min at 37[degrees]C with 1 [micro]L (5 U) of lambda-exonuclease solution (New England Biolabs, Ipswich, MA), with 3 [micro]L exonuclease buffer and 6 [micro]L milli-Q [H.
The study also predicted the presence in the nucleus and cytoplasm of a 5'-to-3' exonuclease that preferentially digests uncapped RNA to 5' mononucleotides exonucleolytically.
It is reported that the Taq DNA polymerase lacks 3`-5` exonuclease activity and therefore, it is unable to cleave the mis-incorporated nucleotides [3].
Structure of bacteriophage T4 RNase H, a 50 to 30 RNA-DNA and DNA-DNA exonuclease with sequence similarity to the RAD2 family of eukaryotic proteins.
In one version of this method, the oligonucleotides are designed to hybridize to the 3' side (“downstream”) of an amplification primer so that the 5'-3' exonuclease activity of a polymerase digests the 5' end of the probe, cleaving off one of the dyes.
The amplified product was purified using Exonuclease I and Shrimp Alkaline Phosphatase (ExoSAP, USB Corporatin) for DNA sequencing.
Detection of specific polymerase chain reaction product by utilizing the 5' [right arrow] 3' exonuclease activity of Thermus aquaticus DNA polymerase.
DNA secondary structure of the released strand stimulates WRN helicase action on forked duplexes without coordinate action of WRN exonuclease.