0056 DEG, differentially expressed gene; MAPK, mitogen activated protein kinase; ErbB, erythroblastic
leukemia viral oncogene homolog; TGF, transforming growth factor; RIG-I, retinoic acid-inducible gene 1; VEGF, vascular endothelial growth factor; Ig, immunoglobulin; ECM, The extracellular matrix.
5] Human genes: BRCA1, breast cancer 1, early onset; BRCA2, breast cancer 2, early onset; APOE, apolipoprotein E; ERBB2 (formerly HER2/Neu), v-erb-b2 avian erythroblastic
leukemia viral oncogene homolog 2; CHD7, chromodomain helicase DNA binding protein 7.
To determine tumor expression of ERBB2 (v-erb-b2 erythroblastic
leukemia viral oncogene homolog 2) in mammary cancer cohort mice, paraffin sections were placed onto Superfrost/ Plus slides (Fisher Scientific, Pittsburgh, PA), and then deparaffinized and cleared.
Increased amounts of the cell cycle proteins cyclin E and p16, amplification and overexpression of the ERBB2 [v-erb-b2 avian erythroblastic
leukemia viral oncogene homolog 2] gene (which encodes the ERBB2 receptor tyrosine kinase), loss of BAF250A production, and altered amounts of the cell adhesion proteins claudin-3, claudin-4, L1CAM (L1 cell adhesion molecule), EpCAM (epithelial cell adhesion molecule), and E-cadherin have also been documented [reviewed in (24)].
Of all the available examples, perhaps HER2/neu (ERBB2 or v-erb-b2 erythroblastic
leukemia viral oncogene homolog 2, neuro/glioblastoma-derived oncogene homolog [avian]) testing and subsequent treatment of breast cancer with drugs such as trastuzumab or lapatinib is the archetype.
Manufactured products include monoclonal & polyclonal antibodies, Lympholyte(TM) cell separation media, complement for tissue typing, Cellect(TM) Immunocolumns and CELLutiuons immortalized Hypothalamic, Motor Neuron, Hippocampal, Cardiac Endothelial, Oligodendrocytic, Erythroblastic
and Ovarian cell lines
We designed highly purified salt-free OliGold primers (Eurogentec, Seraing, Belgium) for the internal control gene hypoxanthine phosphoribosyhransferase 1 (HPRT1) and for target genes estrogen receptor-[alpha] (ESR1), progesterone receptor (PGR), PDZ domain containing 1 (PDZK1), and erb-b2 erythroblastic
leukemia viral oncogene homolog 2 (ERBB2) using Roche Lightcycler software (Roche Diagnostics Belgium, Vilvoorde, Belgium).
The quantitative ability of dPCR has already been applied to monitoring viral load (14) and measuring copy number variations in HER2 (ERBB2, v-erb-b2 avian erythroblastic
leukemia viral oncogene homolog 2) gene status in breast cancer (15).
22-26) Investigation into the genetic changes found in human adenocarcinomas has identified amplification of the v-erb-b2 erythroblastic
leukemia viral oncogene homolog 2 gene ERBB2 (HER2/neu) in mammary carcinoma.
Target prediction indicated that miR-152 can target v-erb-b2 erythroblastic
leukemia viral oncogene homolog 3 (ERBB3).
Common antecedent diseases or factors include MDS (for example, refractory anemia with excess of blasts or refractory cytopenia with multilineage dysplasia), myeloproliferative neoplasms (for example, chronic myelogenous leukemia with erythroblastic
crisis), (13) and exposure to toxins such as benzene.
The HER2 gene [v-erb-b2 erythroblastic
leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2; also known as HER2)]  is amplified and overexpressed in 15%-20% of primary invasive tumors (13).
About 15%-25% of these cases show amplification of the ERBB2 gene [v-erb-b2 erythroblastic
leukemia viral oncogene homolog 2, neuro/ glioblastoma derived oncogene homolog (avian); also known as HER2 (human epidermal growth factor receptor 2)] (2).