By conducting virus isolation, RT-PCR, and serologic examination, we confirmed an outbreak of epidemic myalgia among adults in Yonezawa, Yamagata, Japan, during June-August 2008 was associated with HPeV3 infection.
However, we succeeded in whole-sequence analysis using a stool specimen from case-patient 11, which suggests the failure to isolate HPeV3 was not a result of low viral load in the specimens from the patients with epidemic myalgia.
Our findings show that epidemic myalgia associated with HPeV3 infection might occur among adults, particular among those [approximately equal to] 30-40 years of age.
We postulate that an outbreak of HPeV3 among children was a necessary background condition for the outbreak of epidemic myalgia among adults in 2008.
In conclusion, we document detection of HPeV3 infection among adult patients with epidemic myalgia in Yamagata, Japan, during 2008.