Production of embryoids
and calli from isolated microspores of tomato (Lycopersicon esculentum Mill.
To study spontaneous differentiation 4-day-old embryoid
bodies were plated in tissue culture dishes in standard embryonic stem cell media without LIF while for the neuronal differentiation study embryonic stem cells were subjected to an 8-day induction procedure that consisted of 4 days of culture as aggregates without RA followed by 4 days of culture in the presence of 1 [micro]M RA ('4-/4+').
This was done by taking germ cells from embryoid
bodies that were only four to 10 days old, and exposing them to stimulating growth factor chemicals.
ES-like cells forming typical embryoid
bodies (EBs) after 5 days were transferred to confocal dishes coated with 0.
Callus and embryoid
formation from protoplasts of Helianthus annuus.
Differentiative potential of a mouse parthenogenetic embryonic stem cell line revealed by embryoid
body formation in vitro.
Martin and her colleagues were the first to demonstrate that female pluripotent stem cells contain two active X-chromosomes and can undergo X-inactivation in vitro when they form embryoid
bodies, and she went on to characterize some of the epigenetic events leading to X-inactivation.
ES cells are differentiated into specified cells by embryoid
body (EB) formation and addition of trophic factors, co-culture with feeder cells or others (Assady et al.
The identification of these cells and their successful expansion in the form of embryoid
bodies may lead to the development of new therapeutic strategies that will avoid the use of human embryos.
The LEAP Workstation allows rapid and automated physical passaging of stem cells and consistent embryoid
body generation, in addition to its unique in situ cell and colony purification capabilities.
body formation and in vitro differentiation Evaluation of the differentiation of porcine ES-like cells was conducted by spontaneously prolonged culture and in hanging drops as embryoid
An important addition to Cyntellect's Stem Cell Manager suite of software already consisting of automated passaging and uniform embryoid
body generation tools, this latest application has been developed to address current limitations in stem cell colony purification, and is a major advancement over existing methods.
One version of StemDisc aids in embryoid
body (EB) formation.
The article reports on the researchers' findings of an ultra-rapid fabrication and culture method for embryoid
body (EB) formation utilizing a laser-jet printer to generate closely arrayed honeycomb microwells of tunable sizes for the induction of uniform EBs from single cell suspension.
StemDisc, designed to improve embryoid
body (EB) formation of stem cells at a higher rate and efficiency over current EB formation methods.