1974) and Bradford (1976), respectively, and the expression of the recombinant protein was detected electrophoretically
under denaturing conditions using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) according to Laemmli (1970).
5] The presence of CK-MB above 5% of total CPK, as measured electrophoretically
, has also been employed for definition of contusion.
The amplified products were electrophoretically
separated and visualized in 1.
The total RNA integrity was verified electrophoretically
by ethidium bromide staining and purity was determined by absorption rate relationship of OD260/280.
2] With Itano, a physical chemist Linus Pauling demonstrated electrophoretically
abnormal haemoglobin, HbS as abnormal variant of HbA in 1949.
Abstract--The time-dependent formation of electrophoretically
deposited cadmium selenide (CdSe) films on silicon substrates was investigated.
Table 1: Time course and average raw volumes exhibited by electrophoretically
separated serum protein fractions of myocardial infarction patients.
Restricted fragments were electrophoretically
separated on 2% agarose gel and photodocumented using KODAK Edas Software.
For western blot, electrophoretically
separated proteins were transferred onto nitrocellulose sheets at 300mA for 3hrs at 4[degrees]C.
Concentrations of RNA were measured by spectrophotometry (OD 260 nm), and RNA integrity was electrophoretically
verified using ethidium bromide.
Western blotting was performed on similarly prepared acrylamide gel and electrophoretically
transferred onto the nitrocellulose membrane.
Amplification products were separated electrophoretically
For the immunotransfer technique, once the gel was electrophoretically
fractioned by SDS-PAGE to 10% it was transferred to nitrocellulose membranes (0.
Objectives: To study the freq uencies of two electrophoretically
distinguishable hemoglobin types from Saudi Arabia.
Extension products were purified with DyeEx columns (Qiagen, Hilden, Germany), and separated electrophoretically
and detected in an ABI PRISM 377XL automated sequencer (Applied Biosystems).