Figure 2 shows an SEM image of electrophoretically
deposited (NiCoCrAlY) particles cemented with [Al.
For Western blot, proteins from SDS gels were electrophoretically
transferred to nitrocellulose membranes in a semi-dry apparatus (Sree Maruthi Scientific Works, Bangalore, India) (2 h at 125 mA) (13).
A comparison of electrophoretically
detectable isozyme differences in 6 populations of An.
10] dilutions of the proteinase K-digested samples were electrophoretically
separated on Trisglycine gel and transferred to a nitrocellulose membrane.
This substitution yields the electrophoretically
distinct hemoglobin described by Linus Pauling in 1949 (11).
It was then electrophoretically
deposited onto the surface of 316L SS substrate from a 3% suspension in isopropanol.
Equal amounts (20 lag of protein) of each sample were electrophoretically
separated on a 10% SDS polyacrylamide gel and electro-transferred to a PVDF (polyvinylidene difluoride) membrane.
Specific molecular binding events result in the release of electrophoretically
distinct eTag reporters, which are then resolved by standard capillary electrophoresis to provide precise, sensitive quantitation of multiple analytes -- directly from cell lysates of cultured or primary cells, as well as fresh and fixed tissue samples.
A popular method for measuring enzymatic processes is electrophoretically
mediated microanalysis (EMMA) .
Proteins in the gels were electrophoretically
transferred (60 volts, 1 h) to nitrocellulose paper (0.
Both Bence Jones proteins and B2M share the common characteristic of being low-molecular-weight proteins that may (in the case of Bence Jones proteins) or do (B2M) migrate electrophoretically
in the [Beta] region.
The PCR product is then detected electrophoretically
indicating either the presense or abscense of the disease-causing phytoplasm.
In the bottom, the flow was divided into a number of outlet tubes and the electrophoretically
separated components were supposed to come in different tubes.
Hybond-P membranes (GE Healthcare Life Sciences, Waukesha, WI, USA) containing the electrophoretically
transferred protein bands were probed using the various primary antibodies.
RNA integrity was verified electrophoretically
by ethidium bromide staining, and RNA in samples was quantified.