Amplification products were electrophoresed
on a DGGE gel, and subsequently the mobility of single bands were analyzed.
PCR-amplified DNA fragments were electrophoresed
, prior to and following the cleanup process, on 1% agarose gels in Ix TBE buffer (50 mM Tris-HCl, 50 mM boric acid, and 1 mM EDTA) containing ethidium bromide, and visualized using a ChemiImager[R] 4400 Imaging System (Alpha Innotech, San Leandro, California).
Products were subsequently electrophoresed
across a 1.
amplification, the samples were electrophoresed
Samples were electrophoresed
using SDS-PAGE and autoradiography was obtained.
The gels were electrophoresed
at 40 V for -2 h and photographed.
The scientists further purified and enriched the draining liquid, which was then electrophoresed
Isolates with similar PFGE patterns were restricted and electrophoresed
on the same gel to confirm identity.
If it is necessary to observe shorter fragments, a shorter running time would be needed as fragments less than 450 or so base pairs were run off the bottom of the gel when gels were electrophoresed
for 11 hours.
PCR products were electrophoresed
on 2% agarose gels, stained with ethidium bromide, and visualized under UV light.
T-cell receptor gene rearrangement analysis was performed by PCR in a multiplex reaction according to the method of Theodorou et al, (4) except that no GC clamps were added to the primers during their synthesis, and PCR products were electrophoresed
on a 2.
Two [micro]l of each sample were electrophoresed
in a 1.
for one and one-half hours, stained the gel with ethidium bromide for 10 minutes and viewed and photographed the Hhal fragments with an ultraviolet light source for the gel image photo.
The protein lysate supernatants were electrophoresed
on 15% (w/v) SDSpolyacrylamide gels, and unstained protein molecular weight marker (MBI Fermentas, Pittsburgh, PA, USA) was used as the molecular weight standard.
Aliquots of the PCR products were electrophoresed