The quantification of Ki67 indices in melanomas by DIA may thus require the use of Ki67/ MART1 double stains to accurately segment Ki67-positive melanocytic cells from other proliferating cells.
To our knowledge, no studies have established and explored automated Ki67 indices in melanocytic pathology, for either single or double stains.
We compared automated indices with indices previously obtained by conventional microscopy, and illustrated to what extent accuracy of the Ki67 index was improved when based on Ki67/MART1 double stains as opposed to Ki67 single stains.
6) was established on the Ki67/MART1 double stains without taking the information given by the MART1 stain into account (Figure 1, D).
In melanocytic pathology, novel research favors Ki67/ MART1 double stains to accurately distinguish Ki67-positive melanocytic cells from other proliferating Ki67-positive cells, mainly lymphocytes and stromal and epithelial cells.
We hypothesized that because MUC4 and p53 show staining limited to the cytoplasm and nucleus, respectively, avoiding interference with interpretation, the double stain might combine the strengths of the 2 stains and overcome some of their limitations.
Additionally, 20 consecutive needle core biopsies (10 pancreatic adenocarcinoma and 10 benign pancreas) were identified to further evaluate the double stain for MUC4 and p53 in a more practical setting.
A 2-color, double stain was developed using MUC4 and p53.
A newly developed double stain using MUC4 and p53 was then evaluated because these 2 antibodies show staining limited to the cytoplasm and nucleus, respectively, avoiding interference of interpretation between the stains.