ANS displacement studies have frequently been used to determine dissociation constants
for potential ligands of TTR (Cao et al.
We determined the dissociation constants
for the BL22P1B11-bilirubin and BL22P1B11-Rh-bilirubin complexes by titrating the [B.
This change of the binding properties is quantified by a 10-fold decrease of the enzyme-substrate complex dissociation constants
, which is sufficient for the generation of cooperativity of the enzyme.
Acid 1 (1063 molecules, 2893 pKas) Dissociation Constants
of Organic Acids in Aqueous Solution, by G.
For PE2I this constant is in rather good agreement with the dissociation constant
Base 2 (~4300 molecules) Dissociation Constants
of Organic Bases in Aqueous Solution, Supplement 1972, by D.
We determined the equilibrium dissociation constants
for both the Tacrolimus AM 2 IgG and the Tacrolimus WT mAb IgG using kinetic exclusion assay (KinExA[R]) (Sapidyne Instruments) (23).
Acid-Base Dissociation Constants
in Dipolar Aprotic Solvents.
Table 1 shows the binding dissociation constants
and free energies for enzymes interacting with F-actin in yeast and rabbit muscle.
Determination of Equilibrium Dissociation Constants
Binding of bilirubin to human serum albumin: determination of the dissociation constants
3]H]WAY100635 binding might explain the large discrepancy between the previously determined dissociation constants
(56 pM  and 400 pM ).