The presence of the atypical (A) variant was originally demonstrated by Kalow and colleagues using the local anesthetic dibucaine as an inhibitor.
The standard methods to detect abnormal pseudocholinesterase, such as the dibucaine or fluoride inhibition tests, fail to detect some enzyme variants.
Dibucaine is a competitive inhibitor of BChE and is used to determine an individual's DN, which is the percentage of BChE inhibited by dibucaine.
A method for the detection of atypical forms of human serum cholinesterase; determination of dibucaine numbers.
Such agents include benzocaine, butamben, cocaine, dibucaine
, lidocaine, pramoxine, prilocaine, tetracaine, and dyclonine (a bactericidal and fungicidal local anesthetic used in lozenges and throat sprays for sore throats).
Don't eat ointment: Three toddlers died after consuming small amounts of over-the-counter ointments containing the anesthetic dibucaine, according to a new report.
Dibucaine often is found in topical ointments used to treat sunburn, cuts and hemorrhoids.
Some of the more common ingredients are benzocaine, dibucaine
, lidocaine, pramoxine, and butamben.
A comparative study of the effects of procaine, lidocaine, tetracaine and dibucaine
on the functions and ultrastructure of isolated rat liver mitochondria.
Hence, the identification of individuals with inherited BCHE variants remains within the province of the laboratory because the demonstration of low plasma cholinesterase activity (BCHE) and abnormal enzyme phenotype (defined by responses to dibucaine
and fluoride inhibitors) quantify the degree of impairment and confirm its hereditary basis.
inhibition test can identify such variants.
4) For >30 years, serum BCHE activity, dibucaine
number (DN), and fluoride number (FN) have provided sufficient information to identify most of the known BCHE phenotypes associated with succinylcholine sensitivity .