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D) Colonies stained with crystal violet were destained, and absorbance was measured on a plate reader at 584 nm; values represent the ratio of absorbance to the number of CFUs.
0 mm mature female, collected with holotype (cleared and stained with alcian and alizarin; only cranium, pectoral girdles and axial skeleton, completely destained by September 2012.
Following electrophoresis, the gel was stained overnight with Coomassie Blue R-250 and then destained in the same solution.
Once your gel has been stained and destained you will be able to see how far the DNA molecules have migrated.
The gel plugs were destained and dehydrated by washing three times (for 10 min) with 25 mM N[H.
2 lactic acid : glycerol : water) and destained in lactoglycerol (Abbott and Robson 1981).
Specimens were stained in aceto-carmine, destained in 70% acidulated alcohol until light pink, and then dehydrated in a stepwise manner with 1 h each in the following concentrations of ethanol: 85%, 95%, 100%, and 100% again.
After staining, the gel was destained in 5% acetic acid.
with Syber Green (Invitrogen, USA) 1:10,000 and destained in acetic acid for 15 min.
The gels were left in a nontoxic stain overnight, and all students destained their gels at virtually the same time to visualize the bands and make a match.
Thin and thick blood smears were prepared, fixed with methanol, stained with 10% Giemsa stain for 30 rain, and destained with water (10).
The gel was subject to electrophoresis at 125 V for 60-90 minutes, stained in ethidium bromide for 10 minutes, and destained in fresh distilled water for 20 minutes prior to illumination under UV light.
Gels were stained with Coomassie blue stain, then oven dried and destained.
Following electrophoresis, the gel was stained for 15 minutes in ethidium bromide solution and destained for 10 minutes in distilled water.
The gels were destained in 7% acetic acid and photographed over a light box with Technical Panatomic Film (Kodak).
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