Entamoeba gingivalis, found only in the dental biofilm
and saliva of patients with periodontal disease, corroborates the hypothesis that this protozoan might be involved in the development of periodontal disease, as has been suggested by other studies.
Factors such as time, frequency, and type of food intake, deficiencies in techniques of dental biofilm
control, low levels of fluoride in the oral environment, and low rate of salivary secretion are some of the ecological determinants influencing variations in biofilm.
However, the presently tested 5-day LSO toothpaste treatment successfully reduced MS levels, and most importantly, MS levels remained low and did not return to baseline during subsequent analysis, suggesting a synergist effect between fluoride and the antibacterial constituents present in Lippia sidoides Cham, essential oil, and emphasizing the importance of the mechanical removal of the dental biofilm
in long-term antibacterial effect against Streptococcus mutans.
22,23) Although this may seem to be a paradox, given the importance of dental biofilm
for caries development and the expected role of its removal in caries control, only a fluoride toothpaste is able to replenish the oral cavity with fluoride and therefore impair caries progression even where toothbrushing is not perfect (which is the site of caries development).
The efficiency of education and motivation for oral hygiene in these patients was confirmed in studies reported by Maciel and co-workers  and that a marked reduction in rates of dental biofilm
occurs, mainly due to the assimilation of techniques appropriate to achieving oral hygiene and therefore the acquisition of healthy habits and routines which is in accordance with the present study.
Effect of sucrose on the selection of mutans streptococci and lactobacilli in dental biofilm
formed in situ.
It has long been recognized that dental biofilm
plays a major role in the etiology of gingivitis, periodontitis, and caries.
The clinical assessment form was also used to document the child's oral hygiene, indicating the presence of dental biofilm
Toothbrushing before meals may not make sense if brushing is being performed to remove food debris; however, when the purpose is to remove dental biofilm
, there may be some logic and limited evidence to support brushing before meal consumption.
However, studies in adults show differences in surface energy between restored and unrestored teeth, influencing adhesion of dental biofilm
, which is reflected in the degree of gingival inflammation that may exist around teeth with some type of restoration.
Dental caries is a chronic disease resulting from an association between a necessary factor, accumulation of dental biofilm
(plaque), and exposure to sugars (negative determining factor).
Numerous drugs have been tested for their effect on dental biofilm
formation and maturation.
Clinical studies have shown definite relationship between dental biofilm
and periodontal disease.
Emphasizing frequent preventive or periodontal maintenance therapy and thorough daily oral hygiene practices, possibly including antimicrobial rinses, is important to reduce dental biofilm
and bacterial aspiration and maintain periodontal health.
Because of their role in the process of dental caries disease, some mutans streptococci have been extensively studied regarding their actions in dental biofilm
and their virulence factors, including their acid tolerance and synthesis of glucans (Duarte et al.