Stained bands were analyzed densitometrically
with Cool Saver imaging software (ATTO Corp.
To assess quantitative differences in the level of protein phosphorylation, we analyzed densitometrically
three protein bands, approximately 33, 37, and 56 kD in molecular weight, that were upregulated after antigen challenge in all Western blot experiments.
Gelatinolytic bands were measured densitometrically
with an image analyzer (Cybernetics) (33).
After background subtraction, identified signal intensities of specific product bands were quantified densitometrically
and normalized with that of [beta]-actin and expressed as arbitrary units.
The SPE patterns observed in this series suggest that it is not always possible to identify a M-spike visually or densitometrically
Positive immunoreactive bands were quantified densitometrically
(Leica Q500IW image analysis system) and expressed as ratio of 3-NT, NF-[kappa]B p65 and p-p38 MAPK to [beta]-actin in optical density units.
Gels were scanned densitometrically
(Appraise Densitometer; Beckman) to assess the BJP fraction of the total urine proteins.