The relative expression of PPAR gamma, adiponectin, and leptin in 3T3-L1 adipocytes was quantified densitometrically
using the software LabWorks 4.
1/2] was verified by fluorescent immunodetection and quantified densitometrically
([greater than or equal to] 75 cells/time point), corrected for background, in the nuclear area using Image] software (version 1.
Mandibular BMD was measured densitometrically
from PRs using copper step wedge.
DNA concentrations were determined by measuring the absorbance at 260 nm, or by fractionating DNA aliquots on a gel against known concentrations of 1-DNA standards and quantifying densitometrically
Deposition of silver on the gold NPs amplifies the absorbance signal, which is recorded densitometrically
Blots were analyzed densitometrically
with a UVP EC3 bioimaging system with Labworks 4.
Unlike conventional cemented and non-cemented THR devices, which can lose 20-40% of proximal femoral bone mass within the first 24 months following surgery, Revelation components have been shown densitometrically
to preserve original bone (95%+ at two years and 100% at four years) in primary THR surgeries and to regenerate bone in revision THR surgeries.
The intensities of the bands were quantified densitometrically
using Image J software version 1.
Positive immunoreactive bands were quantified densitometrically
and expressed as the ratio of problem to [beta]-actin in arbitrary units.
The reactive bands were detected by chemiluminescence and signal intensity was densitometrically
quantitated in Western blotting (Image J, version 2.
Protein concentration was analyzed densitometrically
and corrected with values determined on anti-GAPDH blots.