decolourise


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Therefore, the objectives of the present study were (i) to stain the sputum samples in their containers with phenol ammonium sulphate basic fuchsin solution and to decolourise and counter-stain the stained sputum smears for detection of AFB (henceforth called pot method, and (ii) to compare the proportion of AFB positives obtained in pot and standard ZN methods.
After rinsing the slides with a gentle stream of water, 25 per cent sulphuric acid was used to decolourise the smears for 2 to 3 min.