Bacteriocin prevents the crown gall
tumors from forming.
There have been clues that this technique might be used to produce disease resistance in plants, but we were surprised to discover just how well it actually works in the case of crown gall disease.
Crown gall is caused by the common soil bacterium Agrobacterium tumefacians, which has the unique ability to transfer its own DNA into the DNA of the plant it infects in a process known as horizontal gene transfer.
Crown gall tumors can be maintained as undifferentiated masses of tumor cells in tissue cultures without the infecting bacteria and added hormones required for cell culture of normal tissues.
The T-DNA oncogenes produce uncontrolled proliferation of crown gall cells via the production of auxins and cytokinins for the dividing plant cells and specific opines that are secreted as an energy source for the attached bacterium, but mainly for the surrounding aggregation of A.
A list of the approximate dates of key discoveries in the historical record of crown gall disease since about 1900 is shown in Table I.
tumefaciens, is a gram-negative rod that is the causative agent of Crown Gall Disease.
McLaughlin (1991) concluded that the Crown Gall tumor (potato disc) assay could be used as a fairly rapid, inexpensive and reliable prescreen for antitumor activity.
1980) examined the effects of several compounds and plant extracts on Crown Gall tumor formation and found no effects on bacterial viability or on the attachment process.
Often lethal, always enfeebling, crown gall can occur wherever plants are grown.
Don't confuse crown gall with clumsy graft unions, which may make swellings but don't show notable changes in color or texture.
Agrobacterium has been closely studied since 1907 when scientists showed that it is the cause of the plant disease called crown gall.
In the 1970's and 1980's researchers demonstrated that Agrobacterium transfers a portion of its own DNA into the plant's DNA, thereby introducing new genes that lead to crown gall formation.
The winery's 2,000 acres of Estate vineyards are the site of irrigation, crown gall
and integrated pest management research as well as the propagation of virus-free rootstock.
Armillaria mellea) and crown gall
(Agrobacterium tumefaciens), and the cultivars against blight (Xanthomonas campestris), and development of aflatoxin (Aspergillus flavus).