counterstain


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  • noun

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a stain of contrasting color that is used when the principal stain does not show the structure clearly

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References in periodicals archive ?
C, CD68 (immunohistochemistry with hematoxylin counterstain, original magnifications X400).
Constitutive heterochromatin regions (C-bands) were revealed using the method of Summer (1972), using propidium iodide as a counterstain.
Slides were stained with Harris hematoxylin followed by eosin counterstain (Presnell & Schreibman 1997).
After three final washes in PBS, the cells were mounted with Prolong with DAPI (4,6-diamidino-2-phenylindole; Invitrogen) to counterstain for the nuclei.
The cells were then rinsed with dH20, and, if desired, immediately covered with 100 [micro]l methyl green counterstain solution and incubated for 20 minutes.
The present study was undertaken with the objective to stain sputum samples in their containers by 'phenol (10%) ammonium sulphate (4%) basic fuchsin (2%) solution' and to decolourize and counterstain their smears for detection of AFB- (henceforth called pot method) and to compare the smear results of pot method with the standard Ziehl-Neelsen (ZN) method.
Purified methyl green served as a cytoplasmic and nuclear counterstain.
The solvent bleaches gram-negative bacteria, which microbiologists can then detect using a pink counterstain.
4',6-Diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA, USA) containing mounting medium was used to counterstain the nuclei.
The silver and red chromogens combined with a hematoxylin counterstain allow correlation of gene signals with cellular morphology (Figure 2).
Sections were then washed and developed according to manufacturer instructions before counterstain with 1% fast-green FCF for 5 min.
Tissues were embedded in paraffin, sectioned at 5 [micro]m and stained with hematoxylin followed by eosin counterstain.