The major finding of the present study is that RDX binds to the picrotoxin convulsant site of the [GABA.
The "cage convulsants," compounds with cyclical structure, bind at a convulsant binding pocket inside the [GABA.
We tested the functional implications of RDX binding to the picrotoxin convulsant site in the amygdala, a limbic structure that plays a central role in the generation and propagation of seizures (Aroniadou-Anderjaska et al.
In the present study, we found that RDX induces seizures by binding to the picrotoxin convulsant site of the [GABA.
Additionally, we administered this complex formulation by egg microinjection rather than bath exposure to assure consistent internal composition at the time of exposure to the chemical convulsant.
Embryonic exposure to domoic acid increases the susceptibility of zebrafish larvae to the chemical convulsant pentylenetetrazole.
All contaminant formulations were based relative to a standard dose of p,p '-DDE (1 [micro]M), which was previously determined to enhance sensitivity to chemical convulsants and also to accumulate to levels in zebrafish comparable with those in fetal sea lions (Tiedeken and Ramsdell 2009).
In addition to enhancing induced seizure behavior, p,p '-DDE also promotes a unique and readily observed head-shake behavior in response to the two chemical convulsants (Tiedeken and Ramsdell 2009).
The convulsant activity previously observed at high doses was also investigated.
Fisher's exact test was performed to determine significance of other anticonvulsant and convulsant properties results.
Nantenine showed a dose-dependent convulsant activity at doses ranging from 75 to 105 mg/kg.
Similar to what occurred in the interaction with nantenine, phenytoin heightens the actions of convulsant agents, such as bicuculline and picrotoxin, which possess mechanisms involving the reduction of the inhibitory gabaergic transmission (Stone and Javid, 1982).