Bacterial culture was attempted for 246 (96%) cases, and convalescent serum
was available for 109 (43%) cases.
0 are considered equivocal, and further tests (RT-PCR and/or PRNT, or collection of a convalescent serum
sample) should be performed.
As with the HI assay, diagnosis of acute dengue virus infection using the IgG enzyme linked immunosorbent assay (ELISA) requires testing of paired acute and convalescent serum
samples, showing a greater than four-fold rise in antibody titer.
aid worker Rick Sacra were treated with the convalescent serum
Though the pooled convalescent serum
obtained from a few patients might not contain antibodies against the specific antigenic determinant of the tested strains, considerable variations were observed.
The provincial Board of Health decreed that the MRC "should be authorized to prepare a convalescent serum
for use in the treatment of poliomyelitis, and that the investigation of the present epidemic as to the etiology, clinical course, and treatment should be undertaken" by the MRC.
This probably includes an immediate serum sample for IgM or IgG and possibly a convalescent serum
for IgG; it may include a throat swab or urine sample for viral isolation.
An initial convalescent serum
sample drawn on June 11 (16 days after symptom onset) also tested positive for WNV-specific IgM and IgG by ELISA at MTPHL but was not available for testing at CDC-ADL.
Testing of early convalescent serum
demonstrated no evidence of infection with agents that cause tropical febrile illnesses, including VHF.
To develop the WU ELISA and the KI ELISA, we used as positive controls 2 convalescent serum
samples from a child known to be infected with WUPyV and rabbit hyperimmune serum for WUPyV VP1 or KIPyV VP1, and we used as negative controls rabbit preimmune serum and serum derived from pediatric patients ([less than or equal to] 3 years of age).
pestis or a four-fold change in antibody titer to F1 antigen between paired acute and convalescent serum
samples with a least one sample having a titer [greater than or equal to]1:16.
Acute and convalescent serum
samples were also obtained from probable cases.
A convalescent serum
sample was obtained from the implicated donor 45 days after the initial donation.
CDC has revised the laboratory criteria in the SARS case definition to require that convalescent serum
be collected >28 days after symptom onset, instead of >21 days after symptom onset.
Of the remaining 26 probable cases, convalescent serum
specimens for 25 persons have not been submitted to CDC, and one person, who has recovered, has refused further testing.