DNA synthesis in the cells grown in the presence of 1 [micro]g/ml and 5 [micro]g/ml of carvacrol seems to be normal for both growth and differentiation medium since it is known that CO25 myoblasts cease proliferation when they commit to differentiate after contact inhibition
in the growth medium.
The researchers also found that contact inhibition
in naked mole rats is controlled by two genes, p16 and p27, while in humans it is primarily controlled by p27.
Contact inhibition of growth causes normal cells to spread out and grow in flat monolayers.
Lipkin, Martin Rosenberg and their co-workers have shown CIF can restore contact inhibition, serum dependence and anchorage dependence to malignant cells.