8-11) Regardless of the precursor protein, all types of amyloid appear the same by light and electron microscopies and exhibit identical tinctorial characteristics: Congo red
and thioflavins T and S positivity.
Biopsy the affected organ * Congo red
binding and green birefringence under polarized light * Fibril structure on electron microscopy * X-ray crystallography and infrared spectroscopy to confirm the characteristic cross beta-pleated sheet * Biochemical and/or immunohistochemical identification of the major amyloid protein * For patients with the AL type of amyloid: Immunohistochemical staining: demonstration of kappa and lambda chains in plasma cells Molecular genetic methods: differentiation between poly- and monoclonal B cells Step 2.
8) In addition, most of the vacuolated muscle fibers in h-IBM do not stain positively for Congo red
and do not contain ragged red fibers or cytochrome c oxidase-negative fibers.
for amyloid, von Kossa for calcium, and Perl stain for iron are consistently negative.
Pools of extracellular periodic acid-Schiff-positive material, which were negative for crystal violet and Congo red
, were present among the neoplastic cells (Figure 3).
Special stains (periodic acid-Schiff with diastase, Congo red
, and colloidal iron) and presence of polarizable material can help rule out foreign material, such as silicone and polyvinylpyrrolidone (PVP).
began its life as an extremely valuable textile dye--a dye of such importance that it not only revolutionized the textile industry but also resulted in a patent challenge that changed intellectual property law.
The researchers produced porous metal organic frameworks in this research for the elimination of Congo red
that is found in industrial wastewaters, specially in textile industry.
Buhimschi and her colleagues examined the urine of women with preeclampsia and found that at least five proteins routinely aggregated when the researchers added a dye called Congo red
Both markers have significant effects on the color, macroscopic texture, electron microscopic morphology, and crystal structure of the biosynthesized chitin, being red in presence of Congo Red
and blue in the presence of Calcofluor (Bartnickigarcia et al.
31] concluded that the ability of bacteria to bind Congo red
was associated with virulence.
The diagnosis of amyloid is currently based on specific Congo red
staining on a biopsy of an involved organ by light microscopy under polarized light.
Light microscopic examination revealed an eosinophilic amorphous substance, and Congo red
staining demonstrated green birefringence under polarized light.
Although identified microscopically as an extracellular amorphous eosinophilic material with "apple-green" birefringence by Congo red
staining, amyloid is not a chemically distinct entity (1).
Then, put the mixed powders into a test tube (10 mm x 100 mm) with a wettish congo red
test paper located at 2 cm above the sample so that the distance can help to avoid being influenced by HCl which is released from partial PVC decomposition because of high temperature (17).