When a gene is expressed, the genetic information from one strand of DNA is first copied into a complementary RNA
molecule known as messenger RNA (mRNA).
To monitor the hybridization and reading processes, we also included 7 probes complementary to 7 biotinylated DNA sequences [positive hybridization controls added to complementary RNA
(CRNA) before hybridization] selected
Additionally, the placement of dyes on each domain of the circular-shaped oligonucleotides makes them fluorescent probes, referred to as Cyclicon probes, which allow detection of complementary RNA
When DNA in a cell nucleus becomes active, it either replicates (during periods of growth or reproduction) or serves as a template for making complementary RNA
molecules (during normal cell metabolism).
Once inside the cell, this material is processed into short 21-23 nucleotide RNAs termed siRNAs that are used in a sequence-specific manner to recognize and destroy complementary RNA