But while competitors now have more flexibility to devise tests that compete with Myriad's, the exclusion for complementary DNA
means that Myriad retains much of its patent strength around the two BRCA genes.
Thus, the enzyme requires a single strand for a template and a small RNA fragment for the synthesis of a complementary DNA
This homogeneous phase detection assay is based on the simultaneous binding of 2 aptamer-containing oligonucleotides, each linked to a complementary DNA
reporter sequence containing 2 reactive fluorophore precursor molecules.
RNA was extracted from 250 [micro]L virus-infected culture supernatant by using Trizol LS (Invitrogen, Carlsbad, CA, USA), and the complementary DNA
was synthesized with Oligo(dT)15 (Invitrogen) by using SuperScriptII reverse transcriptase (Invitrogen).
Under normal circumstances, nucleic acid sequences that have large stretches of G residues have a tendency to bind together instead of binding to their complementary DNA
strand, making those sequences extremely difficult to design assays to detect.
mRNA are converted into complementary DNA
(cDNA) and labeled red (infected) and green (noninfected).
At the end of the treatment periods, mRNA was extracted from the cells, pooled according to treaunent and cell line, and used to create labeled complementary DNA
Japan's focus on human full complementary DNA
sequencing and SNPs puts it an ideal position for major advances in the future.
Designated as the world's smallest biological computing device by the Guinness World Records, each computational step consists of two complementary DNA
molecules, acting as input and software, spontaneously bonding.
The latest design, described in the journal Proceedings of the National Academy of Sciences, employs two complementary DNA
molecules - an input molecule and a software molecule.
Experiments also show that duplex DNA with mismatched/bulged regions is less stable than completely complementary DNA
announced in early February that they have acquired a collection of nearly 2,000 disease-related, full-length complementary DNA
(cDNA) clones, providing the raw material for proteomics-based drug discovery research.
In a procedure that mimics DNA replication, the sample to be sequenced is used as a template from which complementary DNA
strands are synthesized to varying lengths.
DNA chips for examining gene expression involve longer fragments of synthetic or complementary DNA
Like many jumping genes, the P-element has two short, mirror-image sequences at either end, which are thought to facilitate a splicing reaction with the host's DNA; the host cell papers over the gaps with complementary DNA