Pfizer has the marketing rights to collagenase
clostridium histolyticum in Europe, and Auxilium Pharmaceuticals, Inc.
Intralesional Injection of Collagenase
In-vitro enzymatic degradation was performed using collagenase
, elastase and trypsin enzymes.
In randomized, double-blind controlled studies of patients with advanced Dupuytren's (mean age was the early 60s), and at least one affected joint with a palpable cord and contracture, significantly greater proportions of patients treated with collagenase
clostridium had almost a complete reduction in contractures after up to three injections, compared with those who received placebo.
The skin was minced, followed by incubation with collagenase
(1 mg/ml in DMEM) for 1-2h at 37[degrees]C.
To study the distribution of LPL within the adipose tissue, we also used collagenase
to digest the extracellular matrix.
The biopharmaceutical company, which has developed and licensed injectable collagenase
for three clinical indications, raised $2.
Skin tissue was cut into pieces, incubated for 30 min with collagenase
, and isolated cells were cultured in DMEM media containing 20% serum for about 2-3 weeks.
Immunohistochemically distribution of type IV collagenase
in normal, benign and malignant breast tissue.
In addition to calcium suppression of parathyroid hormone, preventing bone resorption, collagen, acting on the intestinal lymphatic system, may protect collag en from degradation through the inhibition of cytokine-induced release of metalloproteinases, including collagenase
decreased platelet-derived growth factor (PDGF); decreased basic fibroblast growth factor (b-FGF); increased keratan sulfate; increased stromelysin; increased ratio of stromelysin to tissue inhibitor of metalloproteases (TIMP); increased osteocalcin; increased alkaline phosphatase; increased cAMP responsive to hormone challenge; increased urokinase plasminogen activator; increased cartilage oligomeric matrix protein; and increased collagenase
Studies analyzing wound fluid show it to be rich in growth factors, collagenase
, enzymes and other cells crucial to healing.
Colchicine, a therapeutic agent known to increase endogenous collagenase
production, was tried in a group of patients with alcoholic and viral cirrhosis in a double-blind, randomized, placebo-controlled study.
Half-time results showed an extra 3 per cent sales growth from bone-stimulator activities at Exogen, 3M shoulder implants and the Collagenase
wound-healing product, all bought in the last year.
UV contributes to photoaging, enhanced elastin and collagenase
proteins, and skin damage.