To evaluate molecular clonality
, three slices of 10p FFPE tissue were used for extraction of DNA according to the protocol previously described by Maria et al .
However, additional sampling would seem unlikely to alter our basic conclusions, particularly with regard to the level of MLG diversity across the continental range and the extent of clonality
within and among localities.
seems to be an important feature of the central-south populations of P.
Polymerase chain reaction-based clonality
testing in tissue samples with reactive lymphoproliferations: usefulness and pitfalls.
Without further examination of clonality
or origin (i.
These results underscore the fact that the existence of intratumoral heterogeneity requires that inferences of clonality
require that whole genomes of tumors must be sequenced at a much greater depth than that used to identify base-level mutations present in the majority of subclones (Fig.
They cover basic principles of electrophoresis, protein constituents of serum, determining immunoglobulin clonality
and quantifying specific serum proteins, monoclonal gammopathies, urine protein electrophoresis, and a clinical approach.
At the end, an open lung biopsy with thoracotomy for interstitial lung disease and thymectomy were performed, and the pathologic diagnosis was as micronoduler type thymoma, surrounded by monoclonal B cell proliferation approved by molecular clonality
studies and also amyloid accumulation in the lung, which was shown to be AL type of amyloid by mass spectrometry (Figure 2).
Both the polymerase chain reaction (PCR) and fluorescence in situ hybridisation (FISH) identify tiny regions of DNA of interest to establish clonality
for confirmation of malignancy in a lymphoid population, or to look for characteristic translocations, deletions and gene amplifications in the DNA which are specific for certain subtypes of lymphomas.
Safety will be determined by clinical and laboratory observation and grading of adverse events, analysis of T cell repertoire clonality
, and evaluation of HIV isolates for evidence of vector recombination.
studies for the presence of clonal IgH or Ig Kappa gene rearrangement would be useful in differentiating it from a plasmacytoma.
Sydes, MA and Peakall, R: Extensive clonality
in the endangered shrub Haloragodendron lucasii (Haloragaceae) revealed by allozymes and RAPDs.
However, no study has been done to delineate the clonality
of respiratory tract isolates recovered in India employing multilocus sequence typing (MLST).
Papers from the conference are grouped in sections on malignant and early hematopoiesis, mobilization, gene expression in stem cells, plasticity, regulators, clonality
, and self-renewal.
Determination of clonality
of the lesion is felt to be important, as polyclonal EMP tends not to progress, whereas monoclonal EMP can be followed immunologically.