Most of the cases demonstrated a diploid number (disomy) for the chromosome 9 centromere
FISH probes, the most common numeric changes in PIN and prostate carcinoma include gain of chromosome 7, 8, and 10 and loss of chromosome Y.
To investigate the mechanism of cyclin D1 up-regulation in dysplastic nevi, we analyzed 6 dysplastic nevi with greater than 10% cyclin D1-positive cells using fluorescence in situ hybridization and probes specific for the 11q13 locus and for the centromere
of chromosome 11.
ERBB2 and chromosome 17 centromere
studies of ovarian cancer by fluorescence in situ hybridization.
As a result, they have created a chromosome whose long arm consists only of a centromere
-- the pinched region that holds the two halves of a chromosome together - and a telomere, with no intervening genes.
The company will use the NIH grant to develop a rapid, cost-effective and high throughput mini-chromosome system that tests centromere
sequence function in cell, tissues and whole organisms.
55,56) Thus, patients may appear to have tumors with chromosome 17 polysomy but in reality have HER2 amplification with the amplicon extending to the centromere
A hybridization solution containing directly labeled probes of SpectrumGreen for the chromosome 17 centromere
(CEP) and SpectrumOrange for the HER2 gene locus were applied, and the denaturation and hybridization procedures were perfomed in a HYBrite system (Vysis).
We selected 100 well-spread metaphases per animal from 5 slides for scoring the frequencies of cells with gaps and breaks, cells with at least one chromosome showing early separation of the centromere
(early segregation), polyploid, and extremely damaged (i.
As an aid in the diagnosis of systemic rheumatic diseases, the Varelisa Re-Combi ANA Profile is designed for the qualitative determination of 8 antinuclear antibodies, including dsDNA; Sm (B, B', and D); RNP (68 kDa, A, and C); SS-A/Ro (52 kDa and 60 kDa); 55-B/La; Scl-70; Jo-1; and centromere
in individual breakaway microwells using human serum or plasma.
There is also an internal control, where the probe to the centromere
on chromosome 17 is used, which results in a green fluorescent signal.
The use of a probe directed against the centromere
of chromosome 17, as in dual-color FISH, can differentiate between true Her2/neu gene amplification and polysomy of chromosome 17.
In all 50 tumors we performed cytogenetic analysis and FISH using a DNA-specific probe for the centromere
region of chromosome 3 on cultured tumor cells.
3) Nonstandard abbreviations: CENP-B, centromere
protein B; CK19, cytokeratin 19; SSA/52, SSA 52-kDa antigen; SSA/60, SSA 60-kDa antigen; ANA, anti-nuclear antibody antigen; CCP, cyclic citrullinated peptide; RF, rheumatoid factor; dsDNA, double-stranded DNA; ssDNA, single-stranded DNA; U1snRNP, small nuclear ribonucleoprotein U1; Scl-70, topoisomerase 1; Sm, Smith antigen; GST, glutathione S-transferase; HRP, horseradish peroxidase; CCD, charge-coupled device; TBST, Tris-buffered saline-Tween; RA, rheumatoid arthritis; SLE, systemic lupus erythematosus; SS, Sjogren syndrome; PM, polymyositis; and SSc, systemic sclerosis.
The adenosine triphosphate (ATP)-binding cassette rim protein (ABCR) gene consisting of 50 exons, (35) is located on the short arm of chromosome 1 near to the centromere