occurred at the cut ends of the hypocotyls after five days of culture initiation.
Medium + PGRs Average no Average of Callusing
BAP NAA CW of shoots shoot length (cm) MS + 4 0.
In the second passage, the best callusing
was obtained from
1] NAA optimum callusing
was obtained from internodal explants.
Kiranmai & Prathibha Devi [67,68] studied callusing
and organogenesis from cotyledons of normal and mutated sunflower.
The best result in root explants, callusing
(53%) was observed with the combination of 2,4-D (2 mg/l) and KN (0.
IBA alone or in combination with BAP produced the highest callusing
percentage and the best callusing
degree compared to other auxin at the same concentrations.