Effects of blackseed oil on release of t-PA antigen
1, treatment of HUVEC and HUA-EC with increasing concentrations of blackseed oil for 24 h resulted in a concentration-dependent increase of t-PA antigen in the CM of such treated cells.
2, PAI-1 antigen increased significantly in the CM in a concentration-dependent manner, when either HUVEC [a] or HUA-EC [b] were incubated for 24 h with increasing amounts of blackseed oil.
The observation that endothelial cells of different sites (HUVECs and HUA-ECs) respond similarly to blackseed oil confirms the universality of this pattern across different vascular tissues.
These data provide an evidence that blackseed oil can modulate the fibrinolytic potential of HUVEC and HUA-EC in vitro by increasing the expression of t-PA and PAI-1 in these cells.
The historical tradition of blackseed use in medicine is substantial.
Exposure to the volatile oil obtained from blackseed altered the cellular expression of specific polypeptides in Jurkart T lymphoma cells, suggesting that changes in polypeptide expression might play a role in the biological activities attributed to blackseed (Hailat et al.
Blackseed oil was dissolved in DMSO at a concentration of 10-200 mg and stored at -80[degrees]C.
Effects of blackseed oil on the release of t-PA, u-PA and PAI-1 antigen
Blackseed oil and its preparations have demonstrated significant in vitro and in vivo antineoplastic activity (Salomi et al.
This study showed that blackseed oil reduced PAI-1 antigen significantly in the HT1080 cell line in a concentration-dependent manner.