Cross-reactivities relative to BNP(1-32) 100% were, for the N-terminal antiserum
, BNP (2-32) 0.
120 [micro]l of antiserum
was added to 6 ml of agarose solution and mixed with gentle swirling for uniform distribution of antibody and after solidification the wells were prepared.
After blocking with 3% skim milk in PBS, the membrane was incubated with diluted bovine leptin antiserum
(1:200) for 1 h, followed by incubation with biotinylated secondary antibody, i.
is the only available medical antidote against snakebite (Calmette, 1894), it does not provide enough protection against venom-induced hemorrhage, necrosis, or nephrotoxicity, and it often produces adverse hypersensitivity reactions (Corrigan et al.
For this, we produced rabbit antiserum
to HDI-adducted keyhole limpet hemocyanin (HDI-KLH).
There was an initial increase in overall CVs, probably due to incorrect transfer of values from RPPHS by some manufacturers, although differences in antiserum
reactivity with intact and fragmented Cp cannot be ruled out (see "Comment").
Bordet, in 1898, was one of the first researchers to discover one of the properties of antiserum
: that when the antiserum
raised against an animal was added to the serum of that animal, 'the latter first became opaque, and that after a short time a flocculent precipitate fell' (Nuttall 1901a: 788).
As with all immunological techniques, CIEP requires (1) the production and isolation of antibodies (antiserum
) to known plants and animals, (2) the removal and isolation of plant or animal residue from artifacts or sediment, (3) the exposure of the plant or animal residue to a series of antiserum
, and (4) methods for observing the strength of the reaction of the antiserum
to the unknown residue.
The antibodies in rabbit serum, called antiserum
, can then be used to search for deer blood.
2 /PRNewswire/ -- Proteus International PLC and The Central Veterinary Laboratory (CVL), an Agency of the Ministry of Agriculture, Fisheries and Food (MAFF), today announced they have signed an agreement to undertake a parallel study of Proteus' novel Bovine Spongiform Encephalopathy (BSE) antiserum
and immunohistochemical test on cattle brain tissue.
14 tons of human & animal blood products, antiserum
and vaccines, down 29.
However, chicken antiserum
against A/duck/Laos/XBY004/2014(H5N6) showed broader cross-reactivity with some viruses of other influenza A(H5N1) clades, which might indicate some conservation of epitopes.
(1:5000) for 60 min at room temperature and washed with PBS-Tween-20 (1 mL/L).
showed strong reactivity with APM proteins determined by ELISA, and the reactivity could be detected at dilutions in excess of 1:128,000.
Western blotting analysis using polyclonal antiserum
against fowl poxvirus (FWPV) showed differences from FWPV, but a similar antigenic profile between Hawaiian goose pox (HGP) isolate and 2 previous Hawaiian poxvirus isolates were observed.