3 (#) & vit C Sulfa: sulfasalazine; vit C: vitamin C; vit E: vitamin E; DSP: daily sperm production (DSP); AB+: Aniline Blue
positive; * Indicate significant difference from the control group; (#) Indicate significant difference from treated with Sulfa alone
Several tests such as TdT (terminal deoxyribonucleotidyl transferase)-mediated dUTP nick-end labeling (TUNEL) assay, aniline blue
straining, chromomycin A3 and acridine orange (AO) staining have been described to determine the extent of DNA damage.
Callose was extracted by differential centrifugation and analyzed biochemically based on its ability to bind to aniline blue
Nucleus, nucleoli, and cytoplasm stained with aniline blue
Acidic Aniline Blue
Staining (AABS): During histone replacement with protamines and chromatin condensation in spermatogenesis, normal sperm do not stain with aniline blue
, but sperm with defective density or immature sperm absorb the stain (28).