The analysis of polar body 1 already detected 72 % of all aneuploid
eggs, but adding the analysis of the second polar body significantly improved the detection rate up to 89 %.
They also showed that with increased sequence tags there was increased precision of calling a sample aneuploid
Ms Lesser: Have you seen that patients who repeatedly produce aneuploid
embryos are better able to move on to donor eggs or adoption once they acquire this information?
Even though cancer can arise from a set of precise mutations, the majority of malignant tumors possess aneuploid
cells, and the reason for this is still an open question," said Di Carlo, who is also a member of the California NanoSystems Institute at UCLA.
Results of the present study show that occupational exposures to benzene were associated with increased frequencies of aneuploid
sperm for chromosomes X, Y, or 21.
In the next lab period (two weeks after the first), students need to determine the mode of inheritance for the three mutations by examining the in cis F1 progeny, identify any unexpected aneuploid
progeny that arose through NDJ (see below), and transfer the expected progeny to a fresh culture to form the F2.
4A; double trisomy is unlikely as trisomy 1 is extremely rare, obtained from same patient), one was mosaic aneuploid
(monosomy for chromosome 1 in one blastomere out of 48 blastomeres of the embryo, Fig.
series peaks in frequency at 28 and 30, with a second major peak at 35.
1998) found that the restriction fragment length polymorphism locus XksuA1, mapped to 7DL through aneuploid
analysis, is linked to Dn2 at a distance of 9.
However, other triploid bivalves, like the Japanese oyster (Crassostrea gigas) and the pearl oyster (Pinctada fucata), showed a limited number of aneuploid
gametes (Allen & Downing 1990, Komaru & Wada 1994).
Furthermore, in field and greenhouse experiments in which triploids were used to pollinate diploids, a complex mixture of diploid, triploid, tetraploid, and aneuploid
progeny was encountered (Muller 1972; Sterk 1987), although seed set was significantly lower than after cross-pollination by other diploids.
Over time, the aneuploid
peak in TD crosses split into two peaks: one close to 2 n and the other close to 3 n.
Cells with aneuploid
concentrations of DNA are readily differentiated from euploid cells by means of a diplold control, such as human lymphocytes, or chicken or trout RBCS.
The calculation of the proportion of fetal DNA for the aneuploid
samples was based on the overrepresentation of the abnormal chromosome, and the values ranged from 7% to 20% (see Table 2 in the online Data Supplement).