3]) for 30 min, followed 4 hr later by allergen inhalation challenge with standardized and freeze-dried allergen extracts
Single lots of food allergen extracts
from three different commercial sources were compared for their efficacy in evaluating immediate food hypersensitivity.
Offering classical and new techniques, they also cover a number of disciplines as they address the molecular mechanisms of allergy, culture of T-cells from peripheral blood, T-cell lines and human T-cell clones, human T-cell epitopes of allergens, restriction of T-cell responses, short-term culture of CD8 cells and intracellular cytokine staining, processing of certain T-regulatory cells, using monocyte-derives dentritic cells and antigen-presenters in T-cell proliferation and cytokine production, human cytokine responses, human chemokine production, standardization and characterization of allergen extracts
, conjugation of haptens, monoclonal antibodies, air sample assays, hologene assays, microscopic identification of pollens, identification of mast cells, and in-situ hybridization.
Most of these antibodies also bound to the appropriate allergen extracts
immobilized to paper discs (data not shown).
Alternative approaches aimed at increasing the safety of currently available allergen extracts
include oral administration of encapsulated antigen for delayed absorption in the small intestine; this is being pursued by a Danish company.
Moreover, allergen extracts
prepared by different procedures and marketed by different companies vary considerably in composition and content of allergen materials; some may contain irritants and extraneous materials.
SPTs with allergen extracts
are the favoured method of in vivo testing for IgE-mediated sensitivity.
In cases of suspected allergy to indoor and outdoor aeroallergens, skin-prick testing was performed by standard procedures; prick lancets and allergen extracts
were obtained from Allergo-pharma (17).
5,9) The use of nonstandardized allergen extracts
leads to further difficulties.
Contract notice: Supply of four allergen extracts
for specific hypo-sensitization therapy.
Food allergen extracts
were injected into the gastric mucosa, and the injection sites were monitored and videotaped.
The concept of BM4SIT is based on two innovations: replacing current natural allergen extracts
by mutated recombinant allergens having been designed to be hypo-allergenic but hyper-immunogenic, and adding an adjuvant to support rapid and effective induction of an anti-inflammatory immune response.
Staloral(R) 300 is a sublingual solution of allergen extracts
for allergen immunotherapy.
Several recently introduced "BAST analogs" and "second-generation' methods for in vitro allergy testing that involve a variety of techniques, tracers, allergen extracts
, and antibodies provide discordant results because of differences between their assay characteristics, reagents, and threshold criteria.
The allergen extracts
used in the therapy were donated by the pharmaceutical company Greer Laboratories of Lenoir, N.